Local stimulation induced GABAergic response in rat striatal slice preparations

Intracellular recordings on QX-314 injected neurons

T. Kita, Hitoshi Kita, S. T. Kitai

Research output: Contribution to journalArticle

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Abstract

Gamma-aminobutyric acid (GABA)ergic responses evoked by electrical stimulation in the neostriatal slice preparation were studied in neurons injected intracellularly with Na-conductance blocker QX-314. Local stimulation elicited depolarizing postsynaptic potentials (DPSPs) in the QX-314-injected neurons when the membrane potential was morenegative than -60 mV. When DPSPs were minimized by depolirizing current injection in the QX-314-injected neuron, hyperpolarization was clearly observed following local stimulation. The maximum duration of the hyperpolarizing response to strong local stimulation was about 130 ms. The hyperpolarizing response was blocked by the addition of bicuculine or picrotoxin to the Ringer solution. Intracellular Cl- injections produced changes in the pattern of the local stimulations-induced responses; the initial depolarizing response was followed by a relatively large amplitude long duration depolarization. The polarity of the long duration of depolarizing response could not be reversed by depolarizing currents which were normally sufficient to reverse the polarity of DPSPs in the neurons without Cl- injection. The application of pentobarbital enhanced the amplitude and the duration of the hyperpolarizing responses. The revealed potential of the pentobarbital-enhanced response was estimated to be -60 mV. On the basis of their reversal potential, sensitivity to injected Cl-, sensitivity to GABA blockers picrotoxin and bicuculine, and the effect of pentobarbital, these hyperpolarizing responses are shown to be GABAergic Cl- mediated inhibitory postsynaptic potentials (IPSPs).

Original languageEnglish (US)
Pages (from-to)304-310
Number of pages7
JournalBrain Research
Volume360
Issue number1-2
DOIs
StatePublished - Dec 23 1985

Fingerprint

Corpus Striatum
Synaptic Potentials
Pentobarbital
Neurons
Picrotoxin
gamma-Aminobutyric Acid
Injections
Inhibitory Postsynaptic Potentials
Membrane Potentials
Electric Stimulation
QX-314

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Developmental Biology
  • Clinical Neurology

Cite this

Local stimulation induced GABAergic response in rat striatal slice preparations : Intracellular recordings on QX-314 injected neurons. / Kita, T.; Kita, Hitoshi; Kitai, S. T.

In: Brain Research, Vol. 360, No. 1-2, 23.12.1985, p. 304-310.

Research output: Contribution to journalArticle

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