Localization and regulation of low affinity nerve growth factor receptor expression in the rat olfactory system during development and regeneration

Qizhi Gong, Mary S. Bailey, Sarah K. Pixley, Matthew Ennis, W. Liu, Michael T. Shipley

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Abstract

Nerve growth factor (NGF), a classic neurotropic factor, promotes neuronal survival, maintenance, regeneration and differentiation in the peripheral nervous system and parts of the central nervous system. NGF activity is mediated by cell surface bound receptors including the low affinity NGF receptor (LNGFr) which is expressed by some peripheral and central neurons and is present on peripheral nerve Schwann cells during development and regeneration. The olfactory system is a useful model for the study of the role of LNGFr in neuronal development and regeneration. The growth of olfactory axons into the brain begins in the embryo and continue through the first few postnatal weeks. In mature animals there is persistent turnover and generation of olfactory receptor neurons (ORNs) and continuous growth of new axons into the oflfactory bulb. These new axons grow along the preexisting olfactory pathway. In the mature olfactory system, LNGFr has been observed in the glomerular layer of the olfactory bulb, the target of ORNs. However, neither the cellular localization nor the development expression of LNGFr has been characterized. Her, we tested the hypothesis that LNGFr expression is developmentally regulated in the olfactory nerve and is reinduced following injury to the mature olfactory nerve. LNGFr‐immunoreactivity (IR) was first observed in the olfactory mucosa at embryonic day (E)13 and in the olfactory nerve at E14. LNGFr‐IR increased in the nerve during embryonic development, began to decrease at around postnatal day (P)5 and was scarcely detectable in normal adults. The staining patterns suggests that LNGFr is located on the olfactory nerve Schwann cells. Streaks of LNGFr‐IR were present in the adult olfactory nerve. We reasoned that these streaks might represent transient reexpression of LNGFr associated with normal olfactory neurons turnover and replacement. Consistent with this hypothesis, LNGFr was robustly reexpressed in the adult olfactory nerve following lesion of the olfactory epithelium. Starting late in development (E21) and in the adult, LNGFr‐IR was also observed on fibres in deep layers of the olfactory bulb. LNGFr‐IR was also observed in neurons of the nucleus of the diagonal band (NDB) in the basal forebrain. NDB is the sole source of cholingeric afferents of the olfactory bulb. Thus, we tested the hypothesis that LNGFr in the deep layers of the olfactory bulb is located on NDB axons by making lesions of NDB. Following the lesion, LNGFr‐IR disappeared in the deep layers of the olfactory bulb but remained in the glomerular layer. We conclude that LNGFr‐IR is associated with several distinct populations of cells in the olfactory system. This suggests that LNGFr‐IR plays several distinct functional roles in the olfactory system, including support of olfactory axon growth and regeneration and maintenance of cholinergic innervation of the olfactory bulb. © 1994 Wiley‐Liss, Inc.

Original languageEnglish (US)
Pages (from-to)336-348
Number of pages13
JournalJournal of Comparative Neurology
Volume344
Issue number3
DOIs
StatePublished - Jan 1 1994

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Nerve Growth Factor Receptor
Olfactory Nerve
Regeneration
Olfactory Bulb
Septal Nuclei
Axons
Neurons
Olfactory Receptor Neurons
Olfactory Mucosa
Schwann Cells
Nerve Growth Factor
Growth
Maintenance
Olfactory Pathways
Peripheral Nervous System
Cell Surface Receptors
Peripheral Nerves
Cholinergic Agents
Embryonic Development
Embryonic Structures

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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Localization and regulation of low affinity nerve growth factor receptor expression in the rat olfactory system during development and regeneration. / Gong, Qizhi; Bailey, Mary S.; Pixley, Sarah K.; Ennis, Matthew; Liu, W.; Shipley, Michael T.

In: Journal of Comparative Neurology, Vol. 344, No. 3, 01.01.1994, p. 336-348.

Research output: Contribution to journalArticle

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