Localization of preganglionic neurons that innervate choroidal neurons of pterygopalatine ganglion

Sherry Cuthbertson, Mark Ledoux, Seth Jones, Julia Jones, Qihong Zhou, Suzhen Gong, Patrick Ryan, Anton Reiner

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

PURPOSE. The pterygopalatine ganglion (PPG) receives preganglionic input from the superior salivatory nucleus (SSN) of the facial motor complex and is the main source of parasympathetic input to the choroid in mammals. The present study was undertaken to determine in rats the location and neurotransmitters of SSN neurons innervating those PPG neurons that target the choroid and to determine the location and neurotransmitters of the PPG choroidal neurons themselves. METHODS. Retrograde labeling from rat choroid using a fluorescent tracer, in combination with immunofluorescence labeling for nitric oxide synthase (NOS), vasoactive intestinal polypeptide (VIP), and choline acetyltransferase (ChAT), was used to characterize the location and neurotransmitters of choroidal PPG neurons. To identify SSN neurons that innervate the choroidal PPG neurons, the Bartha strain of the retrograde transneuronal tracer pseudorabies virus (PRV-Ba) was injected into rat choroid, and immunolabeling for NOS or ChAT was used to characterize their neurochemistry. RESULTS. Fluorescent retrograde labeling showed that PPG neurons projecting to the choroid contained NOS, VIP, and ChAT and were widely distributed in PPG and its preganglionic root, the greater petrosal nerve. SSN neurons were ChAT+, and a subset of them was found to contain NOS. PRV-Ba transneuronal retrograde labeling revealed that choroidal preganglionic neurons were localized to the rostral medioventral part of the ipsilateral SSN. The choroidal SSN neurons were ChAT+ and appeared largely to correspond to the NOS+ neurons of the SSN. CONCLUSIONS. These results show that preganglionic neurons in rats that are presumed to regulate choroidal blood flow through the PPG reside within the rostral medioventral SSN, and that NOS is a marker for these SSN neurons.

Original languageEnglish (US)
Pages (from-to)3713-3724
Number of pages12
JournalInvestigative Ophthalmology and Visual Science
Volume44
Issue number9
DOIs
StatePublished - Sep 1 2003

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Parasympathetic Ganglia
Neurons
Choline O-Acetyltransferase
Choroid
Nitric Oxide Synthase
Neurotransmitter Agents
Vasoactive Intestinal Peptide
Suid Herpesvirus 1
Neurochemistry

All Science Journal Classification (ASJC) codes

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Localization of preganglionic neurons that innervate choroidal neurons of pterygopalatine ganglion. / Cuthbertson, Sherry; Ledoux, Mark; Jones, Seth; Jones, Julia; Zhou, Qihong; Gong, Suzhen; Ryan, Patrick; Reiner, Anton.

In: Investigative Ophthalmology and Visual Science, Vol. 44, No. 9, 01.09.2003, p. 3713-3724.

Research output: Contribution to journalArticle

Cuthbertson, Sherry ; Ledoux, Mark ; Jones, Seth ; Jones, Julia ; Zhou, Qihong ; Gong, Suzhen ; Ryan, Patrick ; Reiner, Anton. / Localization of preganglionic neurons that innervate choroidal neurons of pterygopalatine ganglion. In: Investigative Ophthalmology and Visual Science. 2003 ; Vol. 44, No. 9. pp. 3713-3724.
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AU - Ledoux, Mark

AU - Jones, Seth

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AU - Zhou, Qihong

AU - Gong, Suzhen

AU - Ryan, Patrick

AU - Reiner, Anton

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N2 - PURPOSE. The pterygopalatine ganglion (PPG) receives preganglionic input from the superior salivatory nucleus (SSN) of the facial motor complex and is the main source of parasympathetic input to the choroid in mammals. The present study was undertaken to determine in rats the location and neurotransmitters of SSN neurons innervating those PPG neurons that target the choroid and to determine the location and neurotransmitters of the PPG choroidal neurons themselves. METHODS. Retrograde labeling from rat choroid using a fluorescent tracer, in combination with immunofluorescence labeling for nitric oxide synthase (NOS), vasoactive intestinal polypeptide (VIP), and choline acetyltransferase (ChAT), was used to characterize the location and neurotransmitters of choroidal PPG neurons. To identify SSN neurons that innervate the choroidal PPG neurons, the Bartha strain of the retrograde transneuronal tracer pseudorabies virus (PRV-Ba) was injected into rat choroid, and immunolabeling for NOS or ChAT was used to characterize their neurochemistry. RESULTS. Fluorescent retrograde labeling showed that PPG neurons projecting to the choroid contained NOS, VIP, and ChAT and were widely distributed in PPG and its preganglionic root, the greater petrosal nerve. SSN neurons were ChAT+, and a subset of them was found to contain NOS. PRV-Ba transneuronal retrograde labeling revealed that choroidal preganglionic neurons were localized to the rostral medioventral part of the ipsilateral SSN. The choroidal SSN neurons were ChAT+ and appeared largely to correspond to the NOS+ neurons of the SSN. CONCLUSIONS. These results show that preganglionic neurons in rats that are presumed to regulate choroidal blood flow through the PPG reside within the rostral medioventral SSN, and that NOS is a marker for these SSN neurons.

AB - PURPOSE. The pterygopalatine ganglion (PPG) receives preganglionic input from the superior salivatory nucleus (SSN) of the facial motor complex and is the main source of parasympathetic input to the choroid in mammals. The present study was undertaken to determine in rats the location and neurotransmitters of SSN neurons innervating those PPG neurons that target the choroid and to determine the location and neurotransmitters of the PPG choroidal neurons themselves. METHODS. Retrograde labeling from rat choroid using a fluorescent tracer, in combination with immunofluorescence labeling for nitric oxide synthase (NOS), vasoactive intestinal polypeptide (VIP), and choline acetyltransferase (ChAT), was used to characterize the location and neurotransmitters of choroidal PPG neurons. To identify SSN neurons that innervate the choroidal PPG neurons, the Bartha strain of the retrograde transneuronal tracer pseudorabies virus (PRV-Ba) was injected into rat choroid, and immunolabeling for NOS or ChAT was used to characterize their neurochemistry. RESULTS. Fluorescent retrograde labeling showed that PPG neurons projecting to the choroid contained NOS, VIP, and ChAT and were widely distributed in PPG and its preganglionic root, the greater petrosal nerve. SSN neurons were ChAT+, and a subset of them was found to contain NOS. PRV-Ba transneuronal retrograde labeling revealed that choroidal preganglionic neurons were localized to the rostral medioventral part of the ipsilateral SSN. The choroidal SSN neurons were ChAT+ and appeared largely to correspond to the NOS+ neurons of the SSN. CONCLUSIONS. These results show that preganglionic neurons in rats that are presumed to regulate choroidal blood flow through the PPG reside within the rostral medioventral SSN, and that NOS is a marker for these SSN neurons.

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