Localization of spectrin in mammalian brain

I. S. Zagon, P. J. McLaughlin, Steven Goodman

Research output: Contribution to journalArticle

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Abstract

Spectrin is a major skeletal component of the erythrocyte membrane and is essential in controlling cell shape and structural stability. The brain has also been found to be rich in an immunoreactive and structural analogue of spectrin. In the present study, spectrin was localized in the mouse brain by indirect immunofluorescence using an antibody to erythrocyte spectrin that cross-reacts specifically with the α and β subunits of brain spectrin. Spectrin antigens were concentrated in neuronal perikarya and cell processes. Synaptic structures and axons were observed to have little detectable spectrin antigen by immunofluorescence methodology. The cell bodies of glia had a less intense immunoreactivity in contrast to neurons, and glial processes and myelin were unstained. Cell nuclei of neural cells were not fluorescent. These results show that (a) spectrin is found in all regions of mammalian brain and its intensity corresponds to neural cell density, (b) different neural cell types contain variable spectrin content, and (c) within a single neural cell, the regional disposition of spectrin varies.

Original languageEnglish (US)
Pages (from-to)3089-3100
Number of pages12
JournalJournal of Neuroscience
Volume4
Issue number12
StatePublished - Dec 1 1984
Externally publishedYes

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Spectrin
Brain
Neuroglia
Antigens
Cell Shape
Erythrocyte Membrane
Indirect Fluorescent Antibody Technique
Myelin Sheath
Cell Nucleus
Fluorescent Antibody Technique
Axons
Cell Count
Erythrocytes
Neurons

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Zagon, I. S., McLaughlin, P. J., & Goodman, S. (1984). Localization of spectrin in mammalian brain. Journal of Neuroscience, 4(12), 3089-3100.

Localization of spectrin in mammalian brain. / Zagon, I. S.; McLaughlin, P. J.; Goodman, Steven.

In: Journal of Neuroscience, Vol. 4, No. 12, 01.12.1984, p. 3089-3100.

Research output: Contribution to journalArticle

Zagon, IS, McLaughlin, PJ & Goodman, S 1984, 'Localization of spectrin in mammalian brain', Journal of Neuroscience, vol. 4, no. 12, pp. 3089-3100.
Zagon IS, McLaughlin PJ, Goodman S. Localization of spectrin in mammalian brain. Journal of Neuroscience. 1984 Dec 1;4(12):3089-3100.
Zagon, I. S. ; McLaughlin, P. J. ; Goodman, Steven. / Localization of spectrin in mammalian brain. In: Journal of Neuroscience. 1984 ; Vol. 4, No. 12. pp. 3089-3100.
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