Localization of tensln in living isells

Jane T. Lln, Iostma I. Apgar, William Mahlow, Shin Lin

Research output: Contribution to journalArticle

Abstract

Tensin, an atin filament capping and binding l)rotein, is localized to various types ofadherens junctions. Cultured cells lransfected wilh chicken cardiao tensin clNA expresse(t a polypeptide of 200 kD that is incorl)orated into focal adhesions. To map the domain associated with the focal adhesion-targeting property of tensin and to study lhe role of tensin in focal onlacl formation in live ceils, we utilized the green fluorescence protein ( AF P ). The full lengt h and various deletions of tensin sequence were inserted into pEFI for transfection into chicken cardiac fibroblasts, leletion of Ihe N-terminal iwo-I bird of sequence that included the two actiri-hinding sites aild the a till filament capping domain did not inhibil the capability for focal adhesion Io(:alizalion. suggesting that the focal adhesion targeting sequence is within lhe (Sterminal third of tensin coding sequence. Further deletion from the terminal that removed the SH2 domain reduced tile local adhesion localization bul did not eliminate it. Time-lapse fluorescence videomicroscopy of liwl coils showed 1hat the re-distribution of tensin in spreading fibroblasts is highly dynamic. Bright fluorescent patches distribute rapidly along lhe lamellipodia in tife spreading metallane. These patches overlap with newly formed memSrane-substrate contacts as they appear as dark plaques when viewed by intlrfmencl rlqleclion microscopy (IRM). These results provide strong evidence lhal lensin allivey participates in tile early events of menlbrane-substrate contacl which leads tl cell spreading and migration.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number9
StatePublished - Dec 1 1997
Externally publishedYes

Fingerprint

Adhesion
Focal Adhesions
Fibroblasts
Tile
Fluorescence
Chickens
Birds
Substrates
Video Microscopy
Pseudopodia
src Homology Domains
Microscopic examination
Sequence Deletion
Cells
Tensins
Cell Movement
Transfection
Peptides
Microscopy
Cultured Cells

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Lln, J. T., Apgar, I. I., Mahlow, W., & Lin, S. (1997). Localization of tensln in living isells. FASEB Journal, 11(9).

Localization of tensln in living isells. / Lln, Jane T.; Apgar, Iostma I.; Mahlow, William; Lin, Shin.

In: FASEB Journal, Vol. 11, No. 9, 01.12.1997.

Research output: Contribution to journalArticle

Lln, JT, Apgar, II, Mahlow, W & Lin, S 1997, 'Localization of tensln in living isells', FASEB Journal, vol. 11, no. 9.
Lln JT, Apgar II, Mahlow W, Lin S. Localization of tensln in living isells. FASEB Journal. 1997 Dec 1;11(9).
Lln, Jane T. ; Apgar, Iostma I. ; Mahlow, William ; Lin, Shin. / Localization of tensln in living isells. In: FASEB Journal. 1997 ; Vol. 11, No. 9.
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