Loss of DNA behind the growing point of thymine-starved Bacillus subtilis 168

Harvard Reiter, Ramareddy Guntaka

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Bacillus subtilis 168 thyA, thyB cells degrade and lose from 10 to 15% of their DNA in four hours of thymine starvation. Degradation appears to be initiated at the DNA replicating point and to continue back along the chromosome. First the newly synthesized strands of DNA are degraded to acid-soluble material, then both the new strands and the template strands are degraded to double-strand segments with sedimentation rates, S020,W, of 6 to 22 s. Cell death is coincident with the loss of DNA. Both start within the first ten minutes of starvation. It is possible that cell death is caused by the irreversible loss of DNA from the region behind the replicating point.

Original languageEnglish (US)
Pages (from-to)533-548
Number of pages16
JournalJournal of Molecular Biology
Volume50
Issue number2
DOIs
StatePublished - Jun 14 1970

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Thymine
Bacillus subtilis
DNA
Starvation
Cell Death
Chromosomes
Acids

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

Loss of DNA behind the growing point of thymine-starved Bacillus subtilis 168. / Reiter, Harvard; Guntaka, Ramareddy.

In: Journal of Molecular Biology, Vol. 50, No. 2, 14.06.1970, p. 533-548.

Research output: Contribution to journalArticle

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