Low-dose steroid alters in vivo endotoxin-induced systemic inflammation but does not influence autonomic dysfunction

Sonia Alvarez, Maria Katsamanis Karavidas, Susette M. Coyle, Shou En Lu, Marie Macor, Leo O. Oikawa, Paul M. Lehrer, Steve E. Calvano, Stephen F. Lowry

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Severe injury and infection are associated with autonomic dysfunction. Diminished heart rate variability (HRV) is also observed as a component of autonomic dysfunction and is induced by endotoxin administration to healthy subjects. It is established that low-dose glucocorticoid administration diminishes the systemic inflammatory manifestations of endotoxinemia but the influence of this anti-inflammatory intervention on overall autonomic dysfunction and HRV responses to endotoxin is unknown. This study was designed to assess the influence of a low-dose hydrocortisone infusion upon endotoxin-elicited systemic inflammatory responses including phenotypic features, cytokine production, and parameters of HRV. Of 19 subjects studied, nine received a continuous infusion of hydrocortisone (3 μg/kg/min continuously over 6 h) prior to intravenous administration of Escherichia coli endotoxin (2 ng/kg, CC-RE, Lot #2) while 10 healthy subjects received only the endotoxin after a 6-h period of saline control infusion. Serial determinations of vital signs, heart rate variability assessments, and cytokine levels were obtained over the subsequent 24 h. Prior cortisol infusion diminished the peak TNF-α (P < 0.01) and IL-6 (P < 0.0001) responses after endotoxin challenge, as compared to saline infusion controls and diminished the peak core temperature response to endotoxin (P < 0.01). In contrast to the influence of cortisol on the above parameters of systemic inflammation, the significant endotoxin-induced decreases in HRV time and frequency domains were not influenced by prior hydrocortisone treatment. Hence, alterations in autonomic dysfunction occur despite hydrocortisone attenuation of other traditional systemic manifestations of endotoxinemia. The maintenance or restoration of autonomic balance is not influenced by glucocorticoid administration.

Original languageEnglish (US)
Pages (from-to)358-368
Number of pages11
JournalJournal of Endotoxin Research
Volume13
Issue number6
DOIs
StatePublished - Dec 1 2007

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Endotoxins
Hydrocortisone
Steroids
Inflammation
Heart Rate
Glucocorticoids
Healthy Volunteers
Cytokines
Vital Signs
Intravenous Administration
Interleukin-6
Anti-Inflammatory Agents
Maintenance
Temperature
Wounds and Injuries
Infection

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Molecular Biology
  • Cell Biology
  • Infectious Diseases

Cite this

Low-dose steroid alters in vivo endotoxin-induced systemic inflammation but does not influence autonomic dysfunction. / Alvarez, Sonia; Katsamanis Karavidas, Maria; Coyle, Susette M.; Lu, Shou En; Macor, Marie; Oikawa, Leo O.; Lehrer, Paul M.; Calvano, Steve E.; Lowry, Stephen F.

In: Journal of Endotoxin Research, Vol. 13, No. 6, 01.12.2007, p. 358-368.

Research output: Contribution to journalArticle

Alvarez, S, Katsamanis Karavidas, M, Coyle, SM, Lu, SE, Macor, M, Oikawa, LO, Lehrer, PM, Calvano, SE & Lowry, SF 2007, 'Low-dose steroid alters in vivo endotoxin-induced systemic inflammation but does not influence autonomic dysfunction', Journal of Endotoxin Research, vol. 13, no. 6, pp. 358-368. https://doi.org/10.1177/0968051907086465
Alvarez, Sonia ; Katsamanis Karavidas, Maria ; Coyle, Susette M. ; Lu, Shou En ; Macor, Marie ; Oikawa, Leo O. ; Lehrer, Paul M. ; Calvano, Steve E. ; Lowry, Stephen F. / Low-dose steroid alters in vivo endotoxin-induced systemic inflammation but does not influence autonomic dysfunction. In: Journal of Endotoxin Research. 2007 ; Vol. 13, No. 6. pp. 358-368.
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