Lowered susceptibility of K-562 cells treated with gamma interferon in serum-free medium to natural killer cell-mediated cytolysis

A. T. Ichiki, D. G. Sugantharaj, C. S. Hudson, David Gerard, M. A. Barnhill, E. G. Bamberger, C. J. Wust, J. E. Fuhr, C. B. Lozzio

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Abstract

K-562 cells grown in serum-free medium were treated with gamma interferon (IFN-γ) and they became significantly less suceptible to natural killer (NK) cell-mediated cytolysis. To examine if this loss in suceptibility was related to induced differentiation events, the presence of various antigens was determined after induction. There was a coincident expression of class I HLA common antigen, although it is not clear if this is a direct causal relationship. The level of the constitutively expressed myelomonocytic antigen, reactive with anti-Leu-M1, was not affected by IFN-γ induction and three normally nonexpressed monocytic antigens, defined by monoclonal antibodies, remained unexpressed. IFN-γ did induce an enhanced expression of IL-2 receptors on K-562 cells after 2 days of treatment but, thereafter, the expression appeared to be suppressed. Electron microscopy of IFN-γ-treated cells revealed the development of increased surface blebbing and electron-dense cytoplasmic inclusions. These ultramicroscopic changes could not be correlated with definitive differentiation events. We suggest that IFN-γ treatment of K-562 cells induces class I HLA expression and morphological changes that may be important to differentiation events that render the cells susceptible to NK-mediated cytolysis.

Original languageEnglish (US)
Pages (from-to)297-304
Number of pages8
JournalNatural Immunity and Cell Growth Regulation
Volume7
Issue number5
StatePublished - Dec 1 1988

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Serum-Free Culture Media
Natural Killer Cells
Interferons
Interferon-gamma
Antigens
Cells
Interleukin-2 Receptors
Histocompatibility Antigens Class I
Electron microscopy
Inclusion Bodies
Blister
HLA Antigens
Monoclonal Antibodies
Electron Microscopy
Electrons

All Science Journal Classification (ASJC) codes

  • Immunology
  • Clinical Biochemistry

Cite this

Ichiki, A. T., Sugantharaj, D. G., Hudson, C. S., Gerard, D., Barnhill, M. A., Bamberger, E. G., ... Lozzio, C. B. (1988). Lowered susceptibility of K-562 cells treated with gamma interferon in serum-free medium to natural killer cell-mediated cytolysis. Natural Immunity and Cell Growth Regulation, 7(5), 297-304.

Lowered susceptibility of K-562 cells treated with gamma interferon in serum-free medium to natural killer cell-mediated cytolysis. / Ichiki, A. T.; Sugantharaj, D. G.; Hudson, C. S.; Gerard, David; Barnhill, M. A.; Bamberger, E. G.; Wust, C. J.; Fuhr, J. E.; Lozzio, C. B.

In: Natural Immunity and Cell Growth Regulation, Vol. 7, No. 5, 01.12.1988, p. 297-304.

Research output: Contribution to journalArticle

Ichiki, AT, Sugantharaj, DG, Hudson, CS, Gerard, D, Barnhill, MA, Bamberger, EG, Wust, CJ, Fuhr, JE & Lozzio, CB 1988, 'Lowered susceptibility of K-562 cells treated with gamma interferon in serum-free medium to natural killer cell-mediated cytolysis', Natural Immunity and Cell Growth Regulation, vol. 7, no. 5, pp. 297-304.
Ichiki, A. T. ; Sugantharaj, D. G. ; Hudson, C. S. ; Gerard, David ; Barnhill, M. A. ; Bamberger, E. G. ; Wust, C. J. ; Fuhr, J. E. ; Lozzio, C. B. / Lowered susceptibility of K-562 cells treated with gamma interferon in serum-free medium to natural killer cell-mediated cytolysis. In: Natural Immunity and Cell Growth Regulation. 1988 ; Vol. 7, No. 5. pp. 297-304.
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