Lysis of Streptococcus mutans by hen egg white lysozyme and inorganic sodium salts

H. Goodman, J. J. Pollock, L. I. Katona, V. J. Iacono, M. I. Cho, Edwin Thomas

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

S. mutans BHT was grown in a synthetic medium containing radioactive thymidine to monitor deoxyribonucleic acid release. Kinetic experiments demonstrated that although lysozyme alone could not liberate deoxyribonucleic acid, cellular deoxyribonucleic acid was liberated from lysozyme-treated cells by addition of low concentrations of inorganic sodium salts. When the salts were tested for their ability to dislodge cell-bound tritiated lysozyme, the extent of the initial release of enzyme by individual anions correlated with the anion potency for deoxyribonucleic acid liberation (SCN - >ClO 4 - >I - >Br - >NO 3 - >Cl - >F - ), although the total amount of lysozyme dislodged did not correspond directly with cell lysis. Differences in the effectivenesses of anions (SCN - ,HCO 3 - , Cl - , and F - ) in potentiating cell lysis could be enhanced or minimized by varying the lysozyme, anion, and bacterial cell concentration. As the anion concentration was increased for each enzyme concentration and cell concentration, the lysis increased, in some cases markedly, until maximum levels of released deoxyribonucleic acid were attained. The maximum levels of lysis for SCN - and HCO 3 - were similar and were greater than those for Cl - and F - . In addition, the maximum levels were observed to increase for each of the anions as the concentration of lysozyme increased.

Original languageEnglish (US)
Pages (from-to)764-774
Number of pages11
JournalJournal of bacteriology
Volume146
Issue number2
StatePublished - Nov 13 1981
Externally publishedYes

Fingerprint

Egg White
Streptococcus mutans
Muramidase
Anions
Salts
Sodium
DNA
Butylated Hydroxytoluene
Enzymes
Thymidine
hen egg lysozyme

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

Cite this

Goodman, H., Pollock, J. J., Katona, L. I., Iacono, V. J., Cho, M. I., & Thomas, E. (1981). Lysis of Streptococcus mutans by hen egg white lysozyme and inorganic sodium salts. Journal of bacteriology, 146(2), 764-774.

Lysis of Streptococcus mutans by hen egg white lysozyme and inorganic sodium salts. / Goodman, H.; Pollock, J. J.; Katona, L. I.; Iacono, V. J.; Cho, M. I.; Thomas, Edwin.

In: Journal of bacteriology, Vol. 146, No. 2, 13.11.1981, p. 764-774.

Research output: Contribution to journalArticle

Goodman, H, Pollock, JJ, Katona, LI, Iacono, VJ, Cho, MI & Thomas, E 1981, 'Lysis of Streptococcus mutans by hen egg white lysozyme and inorganic sodium salts', Journal of bacteriology, vol. 146, no. 2, pp. 764-774.
Goodman H, Pollock JJ, Katona LI, Iacono VJ, Cho MI, Thomas E. Lysis of Streptococcus mutans by hen egg white lysozyme and inorganic sodium salts. Journal of bacteriology. 1981 Nov 13;146(2):764-774.
Goodman, H. ; Pollock, J. J. ; Katona, L. I. ; Iacono, V. J. ; Cho, M. I. ; Thomas, Edwin. / Lysis of Streptococcus mutans by hen egg white lysozyme and inorganic sodium salts. In: Journal of bacteriology. 1981 ; Vol. 146, No. 2. pp. 764-774.
@article{259a0b66525b42cd8ba44b65a31ba315,
title = "Lysis of Streptococcus mutans by hen egg white lysozyme and inorganic sodium salts",
abstract = "S. mutans BHT was grown in a synthetic medium containing radioactive thymidine to monitor deoxyribonucleic acid release. Kinetic experiments demonstrated that although lysozyme alone could not liberate deoxyribonucleic acid, cellular deoxyribonucleic acid was liberated from lysozyme-treated cells by addition of low concentrations of inorganic sodium salts. When the salts were tested for their ability to dislodge cell-bound tritiated lysozyme, the extent of the initial release of enzyme by individual anions correlated with the anion potency for deoxyribonucleic acid liberation (SCN - >ClO 4 - >I - >Br - >NO 3 - >Cl - >F - ), although the total amount of lysozyme dislodged did not correspond directly with cell lysis. Differences in the effectivenesses of anions (SCN - ,HCO 3 - , Cl - , and F - ) in potentiating cell lysis could be enhanced or minimized by varying the lysozyme, anion, and bacterial cell concentration. As the anion concentration was increased for each enzyme concentration and cell concentration, the lysis increased, in some cases markedly, until maximum levels of released deoxyribonucleic acid were attained. The maximum levels of lysis for SCN - and HCO 3 - were similar and were greater than those for Cl - and F - . In addition, the maximum levels were observed to increase for each of the anions as the concentration of lysozyme increased.",
author = "H. Goodman and Pollock, {J. J.} and Katona, {L. I.} and Iacono, {V. J.} and Cho, {M. I.} and Edwin Thomas",
year = "1981",
month = "11",
day = "13",
language = "English (US)",
volume = "146",
pages = "764--774",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "2",

}

TY - JOUR

T1 - Lysis of Streptococcus mutans by hen egg white lysozyme and inorganic sodium salts

AU - Goodman, H.

AU - Pollock, J. J.

AU - Katona, L. I.

AU - Iacono, V. J.

AU - Cho, M. I.

AU - Thomas, Edwin

PY - 1981/11/13

Y1 - 1981/11/13

N2 - S. mutans BHT was grown in a synthetic medium containing radioactive thymidine to monitor deoxyribonucleic acid release. Kinetic experiments demonstrated that although lysozyme alone could not liberate deoxyribonucleic acid, cellular deoxyribonucleic acid was liberated from lysozyme-treated cells by addition of low concentrations of inorganic sodium salts. When the salts were tested for their ability to dislodge cell-bound tritiated lysozyme, the extent of the initial release of enzyme by individual anions correlated with the anion potency for deoxyribonucleic acid liberation (SCN - >ClO 4 - >I - >Br - >NO 3 - >Cl - >F - ), although the total amount of lysozyme dislodged did not correspond directly with cell lysis. Differences in the effectivenesses of anions (SCN - ,HCO 3 - , Cl - , and F - ) in potentiating cell lysis could be enhanced or minimized by varying the lysozyme, anion, and bacterial cell concentration. As the anion concentration was increased for each enzyme concentration and cell concentration, the lysis increased, in some cases markedly, until maximum levels of released deoxyribonucleic acid were attained. The maximum levels of lysis for SCN - and HCO 3 - were similar and were greater than those for Cl - and F - . In addition, the maximum levels were observed to increase for each of the anions as the concentration of lysozyme increased.

AB - S. mutans BHT was grown in a synthetic medium containing radioactive thymidine to monitor deoxyribonucleic acid release. Kinetic experiments demonstrated that although lysozyme alone could not liberate deoxyribonucleic acid, cellular deoxyribonucleic acid was liberated from lysozyme-treated cells by addition of low concentrations of inorganic sodium salts. When the salts were tested for their ability to dislodge cell-bound tritiated lysozyme, the extent of the initial release of enzyme by individual anions correlated with the anion potency for deoxyribonucleic acid liberation (SCN - >ClO 4 - >I - >Br - >NO 3 - >Cl - >F - ), although the total amount of lysozyme dislodged did not correspond directly with cell lysis. Differences in the effectivenesses of anions (SCN - ,HCO 3 - , Cl - , and F - ) in potentiating cell lysis could be enhanced or minimized by varying the lysozyme, anion, and bacterial cell concentration. As the anion concentration was increased for each enzyme concentration and cell concentration, the lysis increased, in some cases markedly, until maximum levels of released deoxyribonucleic acid were attained. The maximum levels of lysis for SCN - and HCO 3 - were similar and were greater than those for Cl - and F - . In addition, the maximum levels were observed to increase for each of the anions as the concentration of lysozyme increased.

UR - http://www.scopus.com/inward/record.url?scp=0019499385&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019499385&partnerID=8YFLogxK

M3 - Article

C2 - 7217017

AN - SCOPUS:0019499385

VL - 146

SP - 764

EP - 774

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 2

ER -