Lysophosphatidylcholine-induced platelet activation and the role of protein-tyrosine kinase and protein kinase C

D. Yang, S. Zeng, W. L. Liu, Z. G. Guo

Research output: Contribution to journalArticle

Abstract

A dual channel aggregator and a dual wavelength fluorophotometer were used to measure platelet aggregation, 5-HT release, cytosolic calcium ([Ca2+](i)) and cytosolic pH (pH(i)) in washed rabbit platelets induced by lysophosphatidylcholine (LysoPC) and to observe effects of protein tyrosine kinase (PTK) inhibitor genistein (Gen) and protein kinase C (PKC) inhibitor staurosporine (Sta) on these events. The results showed that LysoPC (30-300 μmol·L-1) induced platelet aggregation, 5-HT release and [Ca2+](i) increase in a concentration dependent manner, concomitant with pH(i) increase at concentrations above 100 μmol·L-1. Pretreatment of platelets with Gen produced a rightward shift of the aggregation concentration-response curve, AC50 value increased from (100 ± 23) μmol·L-1 to (200 ± 42) μmol·L-1. This shift was associated with reductions in Δ[Ca2+](i) and ΔpH(i) evoked by LysoPC, but no significant effect on 5-HT release, Sta showed significant inhibitory effects on platelet aggregation, 5-HT release and shifts in [Ca2+](i) and pH(i) induced by LysoPC at lower concentrations, but no effect on high concentration LysoPC-stimulated platelet activation. These results indicated that LysoPC induced platelet aggregation and dense granule release via intracellular calcium mobilization and Na+/H+ exchanger activation. The activation of PTK is involved in LysoPC-stimulated aggregation, calcium mobilization and Na-+/H+ exchange in platelet. However, the 5-HT release is not related to PTK activation, PKC also plays an important role in platelet activation induced by LysoPC at lower concentrations. Another PKC-independent pathway might exist to mediate the action of high concentrations of LysoPC.

Original languageEnglish (US)
Pages (from-to)49-52
Number of pages4
JournalChinese Journal of Pharmacology and Toxicology
Volume13
Issue number1
StatePublished - 1999
Externally publishedYes

Fingerprint

Lysophosphatidylcholines
Platelet Activation
Platelets
Protein-Tyrosine Kinases
Protein Kinase C
Chemical activation
Agglomeration
Serotonin
Platelet Aggregation
Staurosporine
Blood Platelets
Genistein
Protein Kinase Inhibitors
Calcium
Sodium-Hydrogen Antiporter
Protein C Inhibitor
Rabbits
Wavelength

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Toxicology

Cite this

Lysophosphatidylcholine-induced platelet activation and the role of protein-tyrosine kinase and protein kinase C. / Yang, D.; Zeng, S.; Liu, W. L.; Guo, Z. G.

In: Chinese Journal of Pharmacology and Toxicology, Vol. 13, No. 1, 1999, p. 49-52.

Research output: Contribution to journalArticle

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