M protein mediated adhesion of M type 24 Streptococcus pyogenes stimulates release of interleukin-6 by HEp-2 tissue culture cells

Harry Courtney, Itzhak Ofek, David L. Hasty

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We investigated the contributions of lipoteichoic acid and M protein to reversible and irreversible adhesion of group A streptococci and the effects of such adhesion on release of interleukin-6. Streptococci in which lipoteichoic acid was masked by the hyaluronate capsule were readily washed from HEp-2 cells, indicating no attachment. Unencapsulated, M-negative streptococci in which lipoteichoic acid was exposed were removed more slowly, indicating loose attachment. Only unencapsulated streptococci that expressed both lipoteichoic acid and M protein remained stably adherent to HEp-2 cells throughout multiple washes. Streptococci expressing both M protein and lipoteichoic acid induced release of interleukin-6 from HEp-2 cells, whereas an isogenic, M-negative mutant failed to induce release of interleukin-6. These data suggest that lipoteichoic acid mediates reversible adhesion and that M protein is required for irreversible adhesion and for inducing release of interleukin-6 from HEp-2 cells.

Original languageEnglish (US)
Pages (from-to)65-70
Number of pages6
JournalFEMS Microbiology Letters
Volume151
Issue number1
DOIs
StatePublished - Jun 1 1997

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Streptococcus pyogenes
Streptococcus
Interleukin-6
Cell Culture Techniques
Proteins
Capsules
lipoteichoic acid

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

M protein mediated adhesion of M type 24 Streptococcus pyogenes stimulates release of interleukin-6 by HEp-2 tissue culture cells. / Courtney, Harry; Ofek, Itzhak; Hasty, David L.

In: FEMS Microbiology Letters, Vol. 151, No. 1, 01.06.1997, p. 65-70.

Research output: Contribution to journalArticle

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