M2 muscarinic receptors in pontine reticular formation of C57BL/6J mouse contribute to rapid eye movement sleep generation

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Abstract

Microinjecting the acetylcholinesterase inhibitor neostigmine into the pontine reticular formation of C57BL/6J (B6) mouse causes a rapid eye movement (REM) sleep-like state. This finding is consistent with similar studies in cat and both sets of data indicate that the REM sleep-like state is caused by increasing levels of endogenous acetylcholine (ACh). Muscarinic cholinergic receptors have been localized to the pontine reticular formation of B6 mouse but no previous studies have examined which of the five muscarinic receptor subtypes participate in cholinergic REM sleep enhancement. This study examined the hypothesis that M2 receptors in pontine reticular formation of B6 mouse contribute to the REM sleep-like state caused by pontine reticular formation administration of neostigmine. B6 mice (n=13) were implanted with electrodes for recording states of sleep and wakefulness and with microinjection cannulae aimed for the pontine reticular formation. States of sleep and wakefulness were recorded for 4 h following pontine reticular formation injection of saline (control) or neostigmine. Experiments designed to gain insight into the muscarinic receptor subtypes mediating REM sleep enhancement involved pontine reticular formation administration of neostigmine after pertussis toxin, neostigmine after methoctramine, and neostigmine after pirenzepine. Pertussis toxin was used to block effects mediated by M2 and M4 receptors. Methoctramine was used to block M2 and M4 receptors, and pirenzepine was used to block M1 and M4 receptors. Pertussis toxin and methoctramine significantly decreased the neostigmine-induced REM sleep-like state. In contrast, pretreatment with pirenzepine did not significantly decrease the REM sleep-like state caused by neostigmine. These results support the interpretation that M2 receptors in the pontine reticular formation of B6 mouse contribute to the generation of REM sleep.

Original languageEnglish (US)
Pages (from-to)821-830
Number of pages10
JournalNeuroscience
Volume126
Issue number4
DOIs
StatePublished - Jun 28 2004

Fingerprint

Muscarinic M2 Receptors
REM Sleep
Neostigmine
Inbred C57BL Mouse
Sleep
Pertussis Toxin
Muscarinic Receptors
Pirenzepine
Wakefulness
Pontine Tegmentum
Implanted Electrodes
Cholinesterase Inhibitors
Microinjections
Cholinergic Receptors
Cholinergic Agents
Acetylcholine
Cats

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

M2 muscarinic receptors in pontine reticular formation of C57BL/6J mouse contribute to rapid eye movement sleep generation. / Coleman, C. G.; Lydic, Ralph; Baghdoyan, Helen.

In: Neuroscience, Vol. 126, No. 4, 28.06.2004, p. 821-830.

Research output: Contribution to journalArticle

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