Macromolecular synthesis in cells infected by frog virus 3. XIII. Cell-free translation of immediate early viral mRNAs

Rajendra Raghow, Dawn B. Willis, Allan Granoff

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

When nuclease-treated reticulocyte extracts were programmed with immediate early frog virus 3 mRNAs isolated from infected cells treated with cycloheximide (D. B. Willis and A. Granoff, Virology 86, 443-453, 1978), at least five polypeptides were synthesized. Polypeptides made in vitro were identified as authentic viral gene products by partial-digest mapping. We conclude therefore that the immediate early RNAs represent genuine viral mRNAs and are not aberrant transcripts produced in response to cycloheximide.

Original languageEnglish (US)
Pages (from-to)495-497
Number of pages3
JournalVirology
Volume100
Issue number2
DOIs
StatePublished - Jan 30 1980
Externally publishedYes

Fingerprint

Ranavirus
Cycloheximide
Messenger RNA
Peptides
Virology
Reticulocytes
Viral Proteins
RNA

All Science Journal Classification (ASJC) codes

  • Virology
  • Infectious Diseases

Cite this

Macromolecular synthesis in cells infected by frog virus 3. XIII. Cell-free translation of immediate early viral mRNAs. / Raghow, Rajendra; Willis, Dawn B.; Granoff, Allan.

In: Virology, Vol. 100, No. 2, 30.01.1980, p. 495-497.

Research output: Contribution to journalArticle

@article{510324c6be6747b9a28d0d58dfaafe07,
title = "Macromolecular synthesis in cells infected by frog virus 3. XIII. Cell-free translation of immediate early viral mRNAs",
abstract = "When nuclease-treated reticulocyte extracts were programmed with immediate early frog virus 3 mRNAs isolated from infected cells treated with cycloheximide (D. B. Willis and A. Granoff, Virology 86, 443-453, 1978), at least five polypeptides were synthesized. Polypeptides made in vitro were identified as authentic viral gene products by partial-digest mapping. We conclude therefore that the immediate early RNAs represent genuine viral mRNAs and are not aberrant transcripts produced in response to cycloheximide.",
author = "Rajendra Raghow and Willis, {Dawn B.} and Allan Granoff",
year = "1980",
month = "1",
day = "30",
doi = "10.1016/0042-6822(80)90541-3",
language = "English (US)",
volume = "100",
pages = "495--497",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Macromolecular synthesis in cells infected by frog virus 3. XIII. Cell-free translation of immediate early viral mRNAs

AU - Raghow, Rajendra

AU - Willis, Dawn B.

AU - Granoff, Allan

PY - 1980/1/30

Y1 - 1980/1/30

N2 - When nuclease-treated reticulocyte extracts were programmed with immediate early frog virus 3 mRNAs isolated from infected cells treated with cycloheximide (D. B. Willis and A. Granoff, Virology 86, 443-453, 1978), at least five polypeptides were synthesized. Polypeptides made in vitro were identified as authentic viral gene products by partial-digest mapping. We conclude therefore that the immediate early RNAs represent genuine viral mRNAs and are not aberrant transcripts produced in response to cycloheximide.

AB - When nuclease-treated reticulocyte extracts were programmed with immediate early frog virus 3 mRNAs isolated from infected cells treated with cycloheximide (D. B. Willis and A. Granoff, Virology 86, 443-453, 1978), at least five polypeptides were synthesized. Polypeptides made in vitro were identified as authentic viral gene products by partial-digest mapping. We conclude therefore that the immediate early RNAs represent genuine viral mRNAs and are not aberrant transcripts produced in response to cycloheximide.

UR - http://www.scopus.com/inward/record.url?scp=0018924888&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018924888&partnerID=8YFLogxK

U2 - 10.1016/0042-6822(80)90541-3

DO - 10.1016/0042-6822(80)90541-3

M3 - Article

VL - 100

SP - 495

EP - 497

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -