Macromolecular synthesis in cells infected by frog virus 3 XIV. Characterization of the methylated nucleotide sequences in viral messenger RNAs

Rajendra Raghow, Allan Granoff

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We have examined the structure and distribution of methylated sequences in frog virus 3 (FV3) messenger RNAs isolated from infected cells. Terminal and internal methylated nucleotides were deduced from the analysis of radiolabeled viral mRNAs by a combination of a variety of enzymatic digestion and fractionation procedures. We found that FV3 mRNAs contain internal, as well as terminal, methylated nucleotide sequences. Analysis of the structural compositions of the cap oligonucleotides revealed striking heterogeneity; all three common cap types (m7GpppN1p, m7Gppp-N1mpN2p, and m7GpppN1mpN2mpN3p; caps 0, I, and II, respectively) were present, the latter two comprising more than 80% of the total. Heterogeneity was further exhibited in the type of methylated base represented in N1 and N2 positions. Although there was a preponderance of purine bases in the N1 position of both types of caps, all four common bases were detected. Similarly, all four bases were also present in the N2 position of type II caps. Quantitative analysis revealed that on the average there are between 4 and 7 m6A residues per early viral mRNA molecule. In contrast, although late mRNAs are terminally blocked and methylated, internal methylation in this mRNA class could not be detected.

Original languageEnglish (US)
Pages (from-to)283-294
Number of pages12
JournalVirology
Volume107
Issue number1
DOIs
StatePublished - Jan 1 1980
Externally publishedYes

Fingerprint

Ranavirus
Viral RNA
Messenger RNA
Oligonucleotides
Methylation
Digestion
Nucleotides

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

Macromolecular synthesis in cells infected by frog virus 3 XIV. Characterization of the methylated nucleotide sequences in viral messenger RNAs. / Raghow, Rajendra; Granoff, Allan.

In: Virology, Vol. 107, No. 1, 01.01.1980, p. 283-294.

Research output: Contribution to journalArticle

@article{025ec2dbd03c47b49f304554696b1992,
title = "Macromolecular synthesis in cells infected by frog virus 3 XIV. Characterization of the methylated nucleotide sequences in viral messenger RNAs",
abstract = "We have examined the structure and distribution of methylated sequences in frog virus 3 (FV3) messenger RNAs isolated from infected cells. Terminal and internal methylated nucleotides were deduced from the analysis of radiolabeled viral mRNAs by a combination of a variety of enzymatic digestion and fractionation procedures. We found that FV3 mRNAs contain internal, as well as terminal, methylated nucleotide sequences. Analysis of the structural compositions of the cap oligonucleotides revealed striking heterogeneity; all three common cap types (m7GpppN1p, m7Gppp-N1mpN2p, and m7GpppN1mpN2mpN3p; caps 0, I, and II, respectively) were present, the latter two comprising more than 80{\%} of the total. Heterogeneity was further exhibited in the type of methylated base represented in N1 and N2 positions. Although there was a preponderance of purine bases in the N1 position of both types of caps, all four common bases were detected. Similarly, all four bases were also present in the N2 position of type II caps. Quantitative analysis revealed that on the average there are between 4 and 7 m6A residues per early viral mRNA molecule. In contrast, although late mRNAs are terminally blocked and methylated, internal methylation in this mRNA class could not be detected.",
author = "Rajendra Raghow and Allan Granoff",
year = "1980",
month = "1",
day = "1",
doi = "10.1016/0042-6822(80)90293-7",
language = "English (US)",
volume = "107",
pages = "283--294",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Macromolecular synthesis in cells infected by frog virus 3 XIV. Characterization of the methylated nucleotide sequences in viral messenger RNAs

AU - Raghow, Rajendra

AU - Granoff, Allan

PY - 1980/1/1

Y1 - 1980/1/1

N2 - We have examined the structure and distribution of methylated sequences in frog virus 3 (FV3) messenger RNAs isolated from infected cells. Terminal and internal methylated nucleotides were deduced from the analysis of radiolabeled viral mRNAs by a combination of a variety of enzymatic digestion and fractionation procedures. We found that FV3 mRNAs contain internal, as well as terminal, methylated nucleotide sequences. Analysis of the structural compositions of the cap oligonucleotides revealed striking heterogeneity; all three common cap types (m7GpppN1p, m7Gppp-N1mpN2p, and m7GpppN1mpN2mpN3p; caps 0, I, and II, respectively) were present, the latter two comprising more than 80% of the total. Heterogeneity was further exhibited in the type of methylated base represented in N1 and N2 positions. Although there was a preponderance of purine bases in the N1 position of both types of caps, all four common bases were detected. Similarly, all four bases were also present in the N2 position of type II caps. Quantitative analysis revealed that on the average there are between 4 and 7 m6A residues per early viral mRNA molecule. In contrast, although late mRNAs are terminally blocked and methylated, internal methylation in this mRNA class could not be detected.

AB - We have examined the structure and distribution of methylated sequences in frog virus 3 (FV3) messenger RNAs isolated from infected cells. Terminal and internal methylated nucleotides were deduced from the analysis of radiolabeled viral mRNAs by a combination of a variety of enzymatic digestion and fractionation procedures. We found that FV3 mRNAs contain internal, as well as terminal, methylated nucleotide sequences. Analysis of the structural compositions of the cap oligonucleotides revealed striking heterogeneity; all three common cap types (m7GpppN1p, m7Gppp-N1mpN2p, and m7GpppN1mpN2mpN3p; caps 0, I, and II, respectively) were present, the latter two comprising more than 80% of the total. Heterogeneity was further exhibited in the type of methylated base represented in N1 and N2 positions. Although there was a preponderance of purine bases in the N1 position of both types of caps, all four common bases were detected. Similarly, all four bases were also present in the N2 position of type II caps. Quantitative analysis revealed that on the average there are between 4 and 7 m6A residues per early viral mRNA molecule. In contrast, although late mRNAs are terminally blocked and methylated, internal methylation in this mRNA class could not be detected.

UR - http://www.scopus.com/inward/record.url?scp=0019220083&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019220083&partnerID=8YFLogxK

U2 - 10.1016/0042-6822(80)90293-7

DO - 10.1016/0042-6822(80)90293-7

M3 - Article

VL - 107

SP - 283

EP - 294

JO - Virology

JF - Virology

SN - 0042-6822

IS - 1

ER -