Macrophage CD14 impacts immune defenses against influenza virus in allergic hosts

Maneesha Palipane, John D. Snyder, Kim S. LeMessurier, Anna K. Schofield, Stacie N. Woolard, Amali Samarasinghe

Research output: Contribution to journalArticle

Abstract

Asthma and influenza are leading causes of worldwide morbidity and mortality. Although these two conditions can co-exist in the same patient, the immune parameters that impact disease outcomes are not fully elucidated. The importance of macrophages to both conditions suggested a role for CD14, a co-receptor for endotoxin, as a regulatory mechanism for innate immune responses during asthma and influenza co-morbidity. Herein, we hypothesized that parameters of influenza morbidity will be reduced in the absence of CD14. Age and gender matched wild-type (WT) and CD14 knock-out (KO) mice were subjected to our validated model of Aspergillus-induced model of asthma and/or influenza. Characteristics of disease pathogenesis were investigated using standard methods in weight loss, flow cytometry, airway resistance, histology, quantitative real-time PCR, and viral titer quantification. The absence of CD14 did not have an impact on morbidity as these mice were equally susceptible to disease with similar airway resistance. Peribronchovascular inflammation and goblet cell content were equivalent between WT and KO mice in asthma alone and asthma and influenza co-morbidity. Co-morbid KO mice had less lymphocytes and eosinophils in the airways although their lung viral burden was equivalent to WT. Inflammatory gene signatures were altered in co-morbid mice in each genotype. CD14 expression on macrophages is necessary for airway inflammation but not for viral pathogenesis in allergic hosts.

Original languageEnglish (US)
Pages (from-to)212-219
Number of pages8
JournalMicrobial Pathogenesis
Volume127
DOIs
StatePublished - Feb 1 2019

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Orthomyxoviridae
Human Influenza
Asthma
Macrophages
Morbidity
Knockout Mice
Airway Resistance
Inflammation
Goblet Cells
Aspergillus
Viral Load
Eosinophils
Innate Immunity
Weight Loss
Real-Time Polymerase Chain Reaction
Histology
Flow Cytometry
Genotype
Lymphocytes
Lung

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Infectious Diseases

Cite this

Macrophage CD14 impacts immune defenses against influenza virus in allergic hosts. / Palipane, Maneesha; Snyder, John D.; LeMessurier, Kim S.; Schofield, Anna K.; Woolard, Stacie N.; Samarasinghe, Amali.

In: Microbial Pathogenesis, Vol. 127, 01.02.2019, p. 212-219.

Research output: Contribution to journalArticle

Palipane, Maneesha ; Snyder, John D. ; LeMessurier, Kim S. ; Schofield, Anna K. ; Woolard, Stacie N. ; Samarasinghe, Amali. / Macrophage CD14 impacts immune defenses against influenza virus in allergic hosts. In: Microbial Pathogenesis. 2019 ; Vol. 127. pp. 212-219.
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