Macrophage migration inhibitory factor in acute lung injury

expression, biomarker, and associations

Li Gao, Carlos Flores, Shwu Fan-Ma, Edmund J. Miller, Jaideep Moitra, Liliana Moreno, Raj Wadgaonkar, Brett Simon, Roy Brower, Jonathan Sevransky, Rubin M. Tuder, James P. Maloney, Marc Moss, Carl Shanholtz, Charles Yates, Gianfranco Meduri, Shui Q. Ye, Kathleen C. Barnes, Joe G.N. Garcia

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

The macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine central to the response to endotoxemia, is a putative biomarker in acute lung injury (ALI). To explore MIF as a molecular target and candidate gene in ALI, the MIF gene and protein expression were examined in murine and canine models of ALI (high tidal volume mechanical ventilation, endotoxin exposure) and in patients with either sepsis or sepsis-induced ALI. MIF gene expression and protein levels were significantly increased in each ALI model, with serum MIF levels significantly higher in patients with either sepsis or ALI compared with healthy controls (African- and European-descent). The association of 8 MIF gene polymorphisms [single-nucleotide polymorphisms (SNPs)] (within a 9.7-kb interval on chromosome 22q11.23) with the development of sepsis and ALI in European-descent and African-descent populations was studied next. Genotyping in 506 DNA samples (sepsis patients, sepsis-associated ALI patients, and healthy controls) revealed haplotypes located in the 3′ end of the MIF gene, but not individual SNPs, associated with sepsis and ALI in both populations. These data, generated via functional genomic and genetic approaches, suggest that MIF is a relevant molecular target in ALI.

Original languageEnglish (US)
Pages (from-to)18-29
Number of pages12
JournalTranslational Research
Volume150
Issue number1
DOIs
StatePublished - Jan 1 2007

Fingerprint

Macrophage Migration-Inhibitory Factors
Acute Lung Injury
Biomarkers
Polymorphism
Genes
Sepsis
Nucleotides
Chromosomes
Gene expression
Endotoxins
Proteins
Association reactions
Cytokines
DNA
Single Nucleotide Polymorphism
Gene Expression
Endotoxemia
Tidal Volume
Artificial Respiration
Haplotypes

All Science Journal Classification (ASJC) codes

  • Public Health, Environmental and Occupational Health
  • Biochemistry, medical
  • Physiology (medical)

Cite this

Gao, L., Flores, C., Fan-Ma, S., Miller, E. J., Moitra, J., Moreno, L., ... Garcia, J. G. N. (2007). Macrophage migration inhibitory factor in acute lung injury: expression, biomarker, and associations. Translational Research, 150(1), 18-29. https://doi.org/10.1016/j.trsl.2007.02.007

Macrophage migration inhibitory factor in acute lung injury : expression, biomarker, and associations. / Gao, Li; Flores, Carlos; Fan-Ma, Shwu; Miller, Edmund J.; Moitra, Jaideep; Moreno, Liliana; Wadgaonkar, Raj; Simon, Brett; Brower, Roy; Sevransky, Jonathan; Tuder, Rubin M.; Maloney, James P.; Moss, Marc; Shanholtz, Carl; Yates, Charles; Meduri, Gianfranco; Ye, Shui Q.; Barnes, Kathleen C.; Garcia, Joe G.N.

In: Translational Research, Vol. 150, No. 1, 01.01.2007, p. 18-29.

Research output: Contribution to journalArticle

Gao, L, Flores, C, Fan-Ma, S, Miller, EJ, Moitra, J, Moreno, L, Wadgaonkar, R, Simon, B, Brower, R, Sevransky, J, Tuder, RM, Maloney, JP, Moss, M, Shanholtz, C, Yates, C, Meduri, G, Ye, SQ, Barnes, KC & Garcia, JGN 2007, 'Macrophage migration inhibitory factor in acute lung injury: expression, biomarker, and associations', Translational Research, vol. 150, no. 1, pp. 18-29. https://doi.org/10.1016/j.trsl.2007.02.007
Gao, Li ; Flores, Carlos ; Fan-Ma, Shwu ; Miller, Edmund J. ; Moitra, Jaideep ; Moreno, Liliana ; Wadgaonkar, Raj ; Simon, Brett ; Brower, Roy ; Sevransky, Jonathan ; Tuder, Rubin M. ; Maloney, James P. ; Moss, Marc ; Shanholtz, Carl ; Yates, Charles ; Meduri, Gianfranco ; Ye, Shui Q. ; Barnes, Kathleen C. ; Garcia, Joe G.N. / Macrophage migration inhibitory factor in acute lung injury : expression, biomarker, and associations. In: Translational Research. 2007 ; Vol. 150, No. 1. pp. 18-29.
@article{bd56087848434e83876d3d59b1566a01,
title = "Macrophage migration inhibitory factor in acute lung injury: expression, biomarker, and associations",
abstract = "The macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine central to the response to endotoxemia, is a putative biomarker in acute lung injury (ALI). To explore MIF as a molecular target and candidate gene in ALI, the MIF gene and protein expression were examined in murine and canine models of ALI (high tidal volume mechanical ventilation, endotoxin exposure) and in patients with either sepsis or sepsis-induced ALI. MIF gene expression and protein levels were significantly increased in each ALI model, with serum MIF levels significantly higher in patients with either sepsis or ALI compared with healthy controls (African- and European-descent). The association of 8 MIF gene polymorphisms [single-nucleotide polymorphisms (SNPs)] (within a 9.7-kb interval on chromosome 22q11.23) with the development of sepsis and ALI in European-descent and African-descent populations was studied next. Genotyping in 506 DNA samples (sepsis patients, sepsis-associated ALI patients, and healthy controls) revealed haplotypes located in the 3′ end of the MIF gene, but not individual SNPs, associated with sepsis and ALI in both populations. These data, generated via functional genomic and genetic approaches, suggest that MIF is a relevant molecular target in ALI.",
author = "Li Gao and Carlos Flores and Shwu Fan-Ma and Miller, {Edmund J.} and Jaideep Moitra and Liliana Moreno and Raj Wadgaonkar and Brett Simon and Roy Brower and Jonathan Sevransky and Tuder, {Rubin M.} and Maloney, {James P.} and Marc Moss and Carl Shanholtz and Charles Yates and Gianfranco Meduri and Ye, {Shui Q.} and Barnes, {Kathleen C.} and Garcia, {Joe G.N.}",
year = "2007",
month = "1",
day = "1",
doi = "10.1016/j.trsl.2007.02.007",
language = "English (US)",
volume = "150",
pages = "18--29",
journal = "Translational Research",
issn = "1931-5244",
publisher = "Mosby Inc.",
number = "1",

}

TY - JOUR

T1 - Macrophage migration inhibitory factor in acute lung injury

T2 - expression, biomarker, and associations

AU - Gao, Li

AU - Flores, Carlos

AU - Fan-Ma, Shwu

AU - Miller, Edmund J.

AU - Moitra, Jaideep

AU - Moreno, Liliana

AU - Wadgaonkar, Raj

AU - Simon, Brett

AU - Brower, Roy

AU - Sevransky, Jonathan

AU - Tuder, Rubin M.

AU - Maloney, James P.

AU - Moss, Marc

AU - Shanholtz, Carl

AU - Yates, Charles

AU - Meduri, Gianfranco

AU - Ye, Shui Q.

AU - Barnes, Kathleen C.

AU - Garcia, Joe G.N.

PY - 2007/1/1

Y1 - 2007/1/1

N2 - The macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine central to the response to endotoxemia, is a putative biomarker in acute lung injury (ALI). To explore MIF as a molecular target and candidate gene in ALI, the MIF gene and protein expression were examined in murine and canine models of ALI (high tidal volume mechanical ventilation, endotoxin exposure) and in patients with either sepsis or sepsis-induced ALI. MIF gene expression and protein levels were significantly increased in each ALI model, with serum MIF levels significantly higher in patients with either sepsis or ALI compared with healthy controls (African- and European-descent). The association of 8 MIF gene polymorphisms [single-nucleotide polymorphisms (SNPs)] (within a 9.7-kb interval on chromosome 22q11.23) with the development of sepsis and ALI in European-descent and African-descent populations was studied next. Genotyping in 506 DNA samples (sepsis patients, sepsis-associated ALI patients, and healthy controls) revealed haplotypes located in the 3′ end of the MIF gene, but not individual SNPs, associated with sepsis and ALI in both populations. These data, generated via functional genomic and genetic approaches, suggest that MIF is a relevant molecular target in ALI.

AB - The macrophage migration inhibitory factor (MIF), a pro-inflammatory cytokine central to the response to endotoxemia, is a putative biomarker in acute lung injury (ALI). To explore MIF as a molecular target and candidate gene in ALI, the MIF gene and protein expression were examined in murine and canine models of ALI (high tidal volume mechanical ventilation, endotoxin exposure) and in patients with either sepsis or sepsis-induced ALI. MIF gene expression and protein levels were significantly increased in each ALI model, with serum MIF levels significantly higher in patients with either sepsis or ALI compared with healthy controls (African- and European-descent). The association of 8 MIF gene polymorphisms [single-nucleotide polymorphisms (SNPs)] (within a 9.7-kb interval on chromosome 22q11.23) with the development of sepsis and ALI in European-descent and African-descent populations was studied next. Genotyping in 506 DNA samples (sepsis patients, sepsis-associated ALI patients, and healthy controls) revealed haplotypes located in the 3′ end of the MIF gene, but not individual SNPs, associated with sepsis and ALI in both populations. These data, generated via functional genomic and genetic approaches, suggest that MIF is a relevant molecular target in ALI.

UR - http://www.scopus.com/inward/record.url?scp=34250656192&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34250656192&partnerID=8YFLogxK

U2 - 10.1016/j.trsl.2007.02.007

DO - 10.1016/j.trsl.2007.02.007

M3 - Article

VL - 150

SP - 18

EP - 29

JO - Translational Research

JF - Translational Research

SN - 1931-5244

IS - 1

ER -