Macrophage-related fibrinolysis in experimental disseminated histoplasmosis

M. L. Tiku, P. C. McNabb, T. B. Tomasi

Research output: Contribution to journalArticle

Abstract

A model of disseminated histoplasmosis in CBA/J mice was developed. Cultures of Histoplasma capsulatum from the spleens of infected mice suggested almost complete clearance of fungi by week 3. Th adherent spleen cells from infected mice showed a 2- to 20-fold increase in fibrinolysis. The increase in activity was maximal around 1 to 2 weeks and disappeared after week 3 of infection, and this paralleled the progressively decreasing number of culturable fungi from the spleen. In vitro coculture of infected spleen cells or nylon wool-purified immune T cells and proteose peptone-induced macrophages resulted in increased fibrinolysis. Peritoneal exudate cells from infected mice also showed increased fibrinolysis. The addition of soluble antigen to an in vitro culture system resulted not only in an increase in fibrinolytic activity of peritoneal exudate cells derived from infected mice but also of proteose peptone-induced macrophages. These observations suggest that spleen and peritoneal macrophages from H. capsulatum-infected mice exhibit increased fibrinolysis which in turn is indicative of macrophage activation. The mechanism of activation occurs as a result of immunologically specific T cell-macrophage interaction and by the action of histoplasma products on the macrophages. The significance of these findings and the role of the plasminogen activator assay in studies of disseminated fungal infection are discussed.

Original languageEnglish (US)
Pages (from-to)641-646
Number of pages6
JournalInfection and Immunity
Volume49
Issue number3
StatePublished - Jan 1 1985

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Histoplasmosis
Fibrinolysis
Histoplasma
Spleen
Macrophages
Exudates and Transudates
Fungi
T-Lymphocytes
Inbred CBA Mouse
Macrophage Activation
Mycoses
Wool
Plasminogen Activators
Nylons
Peritoneal Macrophages
Coculture Techniques
Cell Communication
Antigens
Infection

All Science Journal Classification (ASJC) codes

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Tiku, M. L., McNabb, P. C., & Tomasi, T. B. (1985). Macrophage-related fibrinolysis in experimental disseminated histoplasmosis. Infection and Immunity, 49(3), 641-646.

Macrophage-related fibrinolysis in experimental disseminated histoplasmosis. / Tiku, M. L.; McNabb, P. C.; Tomasi, T. B.

In: Infection and Immunity, Vol. 49, No. 3, 01.01.1985, p. 641-646.

Research output: Contribution to journalArticle

Tiku, ML, McNabb, PC & Tomasi, TB 1985, 'Macrophage-related fibrinolysis in experimental disseminated histoplasmosis', Infection and Immunity, vol. 49, no. 3, pp. 641-646.
Tiku, M. L. ; McNabb, P. C. ; Tomasi, T. B. / Macrophage-related fibrinolysis in experimental disseminated histoplasmosis. In: Infection and Immunity. 1985 ; Vol. 49, No. 3. pp. 641-646.
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