Maintenance of y receptor dimers in epithelial cells depends on interaction with G-protein heterotrimers

Anne Marie Estes, Kathleen McAllen, Michael S. Parker, Renu Sah, Trevor Sweatman, Edwards Park, Ambikaipakan Balasubramaniam, Floyd R. Sallee, Mary W. Walker, Steven L. Parker

Research output: Contribution to journalArticle

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Abstract

Treatment of CHO cells expressing human Y receptors (Y1, Y 2 or Y4 subtype) with pertussis toxin results in a large decrease in functional receptors, with a preferential loss of heteropentameric assemblies of receptor dimers and G-protein trimers. This occurs in parallel to inactivation of the nucleotide site of Gi α subunits, with a half period of about 4 h. The loss could be mainly due to proteolysis at the level of recycling/ perinuclear endosomes, and of receptor completion in the ER, since it is reduced by co-treatment with ammonium chloride, an inhibitor of particulate proteinases. Antagonists do not strongly decrease the heteropentameric fraction. These findings indicate that the upkeep of Y receptor dimers in epithelial cell lines depends on the association of receptor oligomers with functional Gi α subunits. This interaction could use the juxtamembrane helix 8 in the fourth intracellular domain, and could also be supported by the C-terminal helix of the third intracellular loop, as outlined in the companion review (Parker et al., Amino Acids, doi: 10.1007/s00726-010-0616-1, 2010).

Original languageEnglish (US)
Pages (from-to)371-380
Number of pages10
JournalAmino Acids
Volume40
Issue number2
DOIs
StatePublished - Feb 1 2011

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Ammonium Chloride
CHO Cells
Endosomes
Pertussis Toxin
Recycling
GTP-Binding Proteins
Dimers
Proteolysis
Peptide Hydrolases
Nucleotides
Epithelial Cells
Maintenance
Amino Acids
Cell Line
Oligomers
Association reactions

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Estes, A. M., McAllen, K., Parker, M. S., Sah, R., Sweatman, T., Park, E., ... Parker, S. L. (2011). Maintenance of y receptor dimers in epithelial cells depends on interaction with G-protein heterotrimers. Amino Acids, 40(2), 371-380. https://doi.org/10.1007/s00726-010-0642-z

Maintenance of y receptor dimers in epithelial cells depends on interaction with G-protein heterotrimers. / Estes, Anne Marie; McAllen, Kathleen; Parker, Michael S.; Sah, Renu; Sweatman, Trevor; Park, Edwards; Balasubramaniam, Ambikaipakan; Sallee, Floyd R.; Walker, Mary W.; Parker, Steven L.

In: Amino Acids, Vol. 40, No. 2, 01.02.2011, p. 371-380.

Research output: Contribution to journalArticle

Estes, AM, McAllen, K, Parker, MS, Sah, R, Sweatman, T, Park, E, Balasubramaniam, A, Sallee, FR, Walker, MW & Parker, SL 2011, 'Maintenance of y receptor dimers in epithelial cells depends on interaction with G-protein heterotrimers', Amino Acids, vol. 40, no. 2, pp. 371-380. https://doi.org/10.1007/s00726-010-0642-z
Estes, Anne Marie ; McAllen, Kathleen ; Parker, Michael S. ; Sah, Renu ; Sweatman, Trevor ; Park, Edwards ; Balasubramaniam, Ambikaipakan ; Sallee, Floyd R. ; Walker, Mary W. ; Parker, Steven L. / Maintenance of y receptor dimers in epithelial cells depends on interaction with G-protein heterotrimers. In: Amino Acids. 2011 ; Vol. 40, No. 2. pp. 371-380.
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