MAPK interacts with occludin and mediates EGF-induced prevention of tight junction disruption by hydrogen peroxide

Shyamali Basuroy, Ankur Seth, Bertha Elias, Anjaparavanda P. Naren, Radhakrishna Rao

Research output: Contribution to journalArticle

164 Citations (Scopus)

Abstract

The MAPK (mitogen-activated protein kinase) pathway is a major intracellular signalling pathway involved in EGF (epithelial growth factor) receptor-mediated cell growth and differentiation. A novel function of MAPK activity in the mechanism of EGF-mediated protection of TJs (tight junctions) from H2O2 was examined in Caco-2 cell monolayers. EGF-mediated prevention of H2O2-induced increase in paracellular permeability was associated with the prevention of H 2O2-induced Tyr-phosphorylation, Thr-dephosphorylation and cellular redistribution of occludin and ZO-1 (zonula occludin-1). EGF also prevented H2O2-induced disruption of the actin cytoskeleton and the dissociation of occludin and ZO-1 from the actin-rich detergent-insoluble fractions. MEK (MAPK/ERK kinase, where ERK stands for extracellular signal related kinase) inhibitors, PD98059 and U0126, completely blocked these protective effects of EGF on TJs. EGF rapidly increased the levels of phosphorylated MEK (p-MEK) in detergent-soluble fractions and phosphorylated ERK (p-ERK) in detergent-insoluble fractions. p-ERK was co-localized and co-immunoprecipitated with occludin. GST (glutathione S-transferase) pull-down assay showed that the C-terminal tail of occludin binds to p-ERK in Caco-2 cell extracts. Pair-wise binding studies using recombinant proteins demonstrated that ERK1 directly interacts with the C-terminal tail of occludin. Therefore the present study shows that ERK interacts with the C-terminal region of occludin and mediates the prevention of H2O2-induced disruption of TJs by EGF.

Original languageEnglish (US)
Pages (from-to)69-77
Number of pages9
JournalBiochemical Journal
Volume393
Issue number1
DOIs
StatePublished - Jan 1 2006

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Occludin
Tight Junctions
Mitogen-Activated Protein Kinases
Hydrogen Peroxide
Intercellular Signaling Peptides and Proteins
Mitogen-Activated Protein Kinase Kinases
Detergents
Caco-2 Cells
Actins
Phosphotransferases
Phosphorylation
Growth Factor Receptors
Cell growth
Cell Extracts
Glutathione Transferase
Actin Cytoskeleton
Recombinant Proteins
Cell Differentiation
Monolayers
Permeability

All Science Journal Classification (ASJC) codes

  • Biochemistry

Cite this

MAPK interacts with occludin and mediates EGF-induced prevention of tight junction disruption by hydrogen peroxide. / Basuroy, Shyamali; Seth, Ankur; Elias, Bertha; Naren, Anjaparavanda P.; Rao, Radhakrishna.

In: Biochemical Journal, Vol. 393, No. 1, 01.01.2006, p. 69-77.

Research output: Contribution to journalArticle

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