Mapping the Initiator Binding Taf2 Subunit in the Structure of Hydrated Yeast TFIID

Gabor Papai, Manish Tripathi, Christine Ruhlmann, Sebastiaan Werten, Corinne Crucifix, P. Anthony Weil, Patrick Schultz

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The general transcription factor TFIID is a large multisubunit complex required for the transcription of most protein-encoding genes by RNA polymerase II. Taking advantage of a TFIID preparation partially depleted in the initiator-binding Taf2p subunit, we determined the conformational and biochemical variations of the complex by electron tomography and cryo-electron microscopy of single molecules. Image analysis revealed the extent of conformational flexibility of the complex and the selection of the most homogeneous TFIID subpopulation allowed us to determine an improved structural model at 23Å resolution. This study also identified two subpopulations of Taf2p-containing and Taf2p-depleted TFIID molecules. By comparing these two TFIID species we could infer the position of Taf2p, which was confirmed by immunolabeling using a subunit-specific antibody. Mapping the position of this crucial subunit in the vicinity of Taf1p and of TBP sheds new light on its role in promoter recognition.

Original languageEnglish (US)
Pages (from-to)363-373
Number of pages11
JournalStructure
Volume17
Issue number3
DOIs
StatePublished - Mar 11 2009

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Transcription Factor TFIID
Yeasts
Electron Microscope Tomography
General Transcription Factors
Cryoelectron Microscopy
RNA Polymerase II
Structural Models
Antibodies
Proteins

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Cite this

Papai, G., Tripathi, M., Ruhlmann, C., Werten, S., Crucifix, C., Weil, P. A., & Schultz, P. (2009). Mapping the Initiator Binding Taf2 Subunit in the Structure of Hydrated Yeast TFIID. Structure, 17(3), 363-373. https://doi.org/10.1016/j.str.2009.01.006

Mapping the Initiator Binding Taf2 Subunit in the Structure of Hydrated Yeast TFIID. / Papai, Gabor; Tripathi, Manish; Ruhlmann, Christine; Werten, Sebastiaan; Crucifix, Corinne; Weil, P. Anthony; Schultz, Patrick.

In: Structure, Vol. 17, No. 3, 11.03.2009, p. 363-373.

Research output: Contribution to journalArticle

Papai, G, Tripathi, M, Ruhlmann, C, Werten, S, Crucifix, C, Weil, PA & Schultz, P 2009, 'Mapping the Initiator Binding Taf2 Subunit in the Structure of Hydrated Yeast TFIID', Structure, vol. 17, no. 3, pp. 363-373. https://doi.org/10.1016/j.str.2009.01.006
Papai, Gabor ; Tripathi, Manish ; Ruhlmann, Christine ; Werten, Sebastiaan ; Crucifix, Corinne ; Weil, P. Anthony ; Schultz, Patrick. / Mapping the Initiator Binding Taf2 Subunit in the Structure of Hydrated Yeast TFIID. In: Structure. 2009 ; Vol. 17, No. 3. pp. 363-373.
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