Marine lipopeptide Iturin A inhibits Akt mediated GSK3β and FoxO3a signaling and triggers apoptosis in breast cancer

Goutam Dey, Rashmi Bharti, Gunaseelan Dhanarajan, Subhasis Das, Kaushik Kumar Dey, Prashanth Kumar Bhusetty Nagesh, Ramkrishna Sen, Mahitosh Mandal

Research output: Contribution to journalArticle

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Abstract

Akt kinase is a critical component of the PI3K/Akt signaling pathway, which is frequently over expressed in human cancers including breast. Therapeutic regimens for inhibiting breast cancer with aberrant Akt activity are essential. Here, we evaluated antitumor effect of a marine bacteria derived lipopeptide 'Iturin A' on human breast cancer in vitro and in vivo through disrupting Akt pathway. Proliferation of MDA-MB-231 and MCF-7 breast cancer cells were significantly inhibited by Iturin A and it induced apoptosis as confirmed by increased Sub G1 populations, DNA fragmentation, morphological changes and western blot analysis. Furthermore, Iturin A inhibited EGF induced Akt phosphorylation (Ser473 and Thr308) and its downstream targets GSK3β and FoxO3a. Iturin A inactivated MAPK as well as Akt kinase leading to the translocation of FoxO3a to the nucleus. Gene silencing of Akt in MDA-MB-231 and MCF-7 cells reduced the sensitivity of cancer cells to Iturin A. Interestingly, overexpression of Akt with Akt plasmid in cancer cells caused highly susceptible to induce apoptosis by Iturin A treatment. In a xenograft model, Iturin A inhibited tumor growth with reduced expressions of Ki-67, CD-31, P-Akt, P-GSK3β, P-FoxO3a and P-MAPK. Collectively, these findings imply that Iturin A has potential anticancer effect on breast cancer.

Original languageEnglish (US)
Article number10316
JournalScientific Reports
Volume5
DOIs
StatePublished - May 14 2015

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Lipopeptides
Apoptosis
Breast Neoplasms
Phosphotransferases
Neoplasms
iturin A
MCF-7 Cells
Gene Silencing
DNA Fragmentation
Phosphatidylinositol 3-Kinases
Epidermal Growth Factor
Heterografts
Plasmids
Western Blotting
Phosphorylation
Bacteria

All Science Journal Classification (ASJC) codes

  • General

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Marine lipopeptide Iturin A inhibits Akt mediated GSK3β and FoxO3a signaling and triggers apoptosis in breast cancer. / Dey, Goutam; Bharti, Rashmi; Dhanarajan, Gunaseelan; Das, Subhasis; Dey, Kaushik Kumar; Bhusetty Nagesh, Prashanth Kumar; Sen, Ramkrishna; Mandal, Mahitosh.

In: Scientific Reports, Vol. 5, 10316, 14.05.2015.

Research output: Contribution to journalArticle

Dey, Goutam ; Bharti, Rashmi ; Dhanarajan, Gunaseelan ; Das, Subhasis ; Dey, Kaushik Kumar ; Bhusetty Nagesh, Prashanth Kumar ; Sen, Ramkrishna ; Mandal, Mahitosh. / Marine lipopeptide Iturin A inhibits Akt mediated GSK3β and FoxO3a signaling and triggers apoptosis in breast cancer. In: Scientific Reports. 2015 ; Vol. 5.
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