Maternal obesity is not associated with placental lipid accumulation in women with high omega-3 fatty acid levels

Fernanda L. Alvarado, Virtu Calabuig-Navarro, Maricela Haghiac, Michelle Puchowicz, Pai Jong S. Tsai, Perrie O'Tierney-Ginn

Research output: Contribution to journalArticle

Abstract

Introduction: Placentas of obese women have higher lipid content compared to lean women. We have previously shown that supplementation of overweight and obese women with omega-3 fatty acids decreases placental esterification pathways and total lipid content in a mid-western population (Ohio). We hypothesized that placental lipid accumulation and inflammation would be similar between lean and obese women living in a region of high omega-3 intake, such as Hawaii. Methods: Fifty-five healthy, normal glucose tolerant women from Honolulu Hawaii, dichotomized based on pre-pregnancy BMI into lean (BMI <25 kg/m 2 , n = 29) and obese (BMI >30 kg/m 2 , n = 26), were recruited at scheduled term cesarean delivery. Maternal plasma DHA levels were analyzed by mass spectrometry. Expression of key genes involved in fatty acid oxidation and esterification were measured in placental tissue using qPCR. Total lipids were extracted from placental tissue via the Folch method. TNF-α concentration was measured by enzyme-linked immunosorbent assay in placental lysates. Results: DHA levels were higher in lean women compared to obese women (P = 0.02). However, DHA levels in obese women in Hawaii were eight times higher compared to obese Ohioan women (P=<0.0001). Placental lipid content and expression of key genes involved in fatty acid oxidation and esterification were similar (P > 0.05) between lean and obese women in Hawaii. Furthermore, TNF-α placental lysates were not different between lean and obese women. Conclusions: Though obese women in Hawaii have lower DHA levels compared to their lean counterparts, these levels remain over eight times as high as obese Ohioan women. These relatively high plasma omega-3 levels in obese women in Hawaii may suppress placental lipid esterification/storage and inflammation to the same levels of lean women, as seen previously in vitro.

Original languageEnglish (US)
Pages (from-to)96-101
Number of pages6
JournalPlacenta
Volume69
DOIs
StatePublished - Sep 1 2018

Fingerprint

Omega-3 Fatty Acids
Obesity
Mothers
Lipids
Esterification
Inflammation
Placenta
Mass Spectrometry

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Obstetrics and Gynecology
  • Developmental Biology

Cite this

Maternal obesity is not associated with placental lipid accumulation in women with high omega-3 fatty acid levels. / Alvarado, Fernanda L.; Calabuig-Navarro, Virtu; Haghiac, Maricela; Puchowicz, Michelle; Tsai, Pai Jong S.; O'Tierney-Ginn, Perrie.

In: Placenta, Vol. 69, 01.09.2018, p. 96-101.

Research output: Contribution to journalArticle

Alvarado, Fernanda L. ; Calabuig-Navarro, Virtu ; Haghiac, Maricela ; Puchowicz, Michelle ; Tsai, Pai Jong S. ; O'Tierney-Ginn, Perrie. / Maternal obesity is not associated with placental lipid accumulation in women with high omega-3 fatty acid levels. In: Placenta. 2018 ; Vol. 69. pp. 96-101.
@article{d05fea2cca5b4d0ea719ab0bf6b7ec93,
title = "Maternal obesity is not associated with placental lipid accumulation in women with high omega-3 fatty acid levels",
abstract = "Introduction: Placentas of obese women have higher lipid content compared to lean women. We have previously shown that supplementation of overweight and obese women with omega-3 fatty acids decreases placental esterification pathways and total lipid content in a mid-western population (Ohio). We hypothesized that placental lipid accumulation and inflammation would be similar between lean and obese women living in a region of high omega-3 intake, such as Hawaii. Methods: Fifty-five healthy, normal glucose tolerant women from Honolulu Hawaii, dichotomized based on pre-pregnancy BMI into lean (BMI <25 kg/m 2 , n = 29) and obese (BMI >30 kg/m 2 , n = 26), were recruited at scheduled term cesarean delivery. Maternal plasma DHA levels were analyzed by mass spectrometry. Expression of key genes involved in fatty acid oxidation and esterification were measured in placental tissue using qPCR. Total lipids were extracted from placental tissue via the Folch method. TNF-α concentration was measured by enzyme-linked immunosorbent assay in placental lysates. Results: DHA levels were higher in lean women compared to obese women (P = 0.02). However, DHA levels in obese women in Hawaii were eight times higher compared to obese Ohioan women (P=<0.0001). Placental lipid content and expression of key genes involved in fatty acid oxidation and esterification were similar (P > 0.05) between lean and obese women in Hawaii. Furthermore, TNF-α placental lysates were not different between lean and obese women. Conclusions: Though obese women in Hawaii have lower DHA levels compared to their lean counterparts, these levels remain over eight times as high as obese Ohioan women. These relatively high plasma omega-3 levels in obese women in Hawaii may suppress placental lipid esterification/storage and inflammation to the same levels of lean women, as seen previously in vitro.",
author = "Alvarado, {Fernanda L.} and Virtu Calabuig-Navarro and Maricela Haghiac and Michelle Puchowicz and Tsai, {Pai Jong S.} and Perrie O'Tierney-Ginn",
year = "2018",
month = "9",
day = "1",
doi = "10.1016/j.placenta.2018.07.016",
language = "English (US)",
volume = "69",
pages = "96--101",
journal = "Placenta",
issn = "0143-4004",
publisher = "W.B. Saunders Ltd",

}

TY - JOUR

T1 - Maternal obesity is not associated with placental lipid accumulation in women with high omega-3 fatty acid levels

AU - Alvarado, Fernanda L.

AU - Calabuig-Navarro, Virtu

AU - Haghiac, Maricela

AU - Puchowicz, Michelle

AU - Tsai, Pai Jong S.

AU - O'Tierney-Ginn, Perrie

PY - 2018/9/1

Y1 - 2018/9/1

N2 - Introduction: Placentas of obese women have higher lipid content compared to lean women. We have previously shown that supplementation of overweight and obese women with omega-3 fatty acids decreases placental esterification pathways and total lipid content in a mid-western population (Ohio). We hypothesized that placental lipid accumulation and inflammation would be similar between lean and obese women living in a region of high omega-3 intake, such as Hawaii. Methods: Fifty-five healthy, normal glucose tolerant women from Honolulu Hawaii, dichotomized based on pre-pregnancy BMI into lean (BMI <25 kg/m 2 , n = 29) and obese (BMI >30 kg/m 2 , n = 26), were recruited at scheduled term cesarean delivery. Maternal plasma DHA levels were analyzed by mass spectrometry. Expression of key genes involved in fatty acid oxidation and esterification were measured in placental tissue using qPCR. Total lipids were extracted from placental tissue via the Folch method. TNF-α concentration was measured by enzyme-linked immunosorbent assay in placental lysates. Results: DHA levels were higher in lean women compared to obese women (P = 0.02). However, DHA levels in obese women in Hawaii were eight times higher compared to obese Ohioan women (P=<0.0001). Placental lipid content and expression of key genes involved in fatty acid oxidation and esterification were similar (P > 0.05) between lean and obese women in Hawaii. Furthermore, TNF-α placental lysates were not different between lean and obese women. Conclusions: Though obese women in Hawaii have lower DHA levels compared to their lean counterparts, these levels remain over eight times as high as obese Ohioan women. These relatively high plasma omega-3 levels in obese women in Hawaii may suppress placental lipid esterification/storage and inflammation to the same levels of lean women, as seen previously in vitro.

AB - Introduction: Placentas of obese women have higher lipid content compared to lean women. We have previously shown that supplementation of overweight and obese women with omega-3 fatty acids decreases placental esterification pathways and total lipid content in a mid-western population (Ohio). We hypothesized that placental lipid accumulation and inflammation would be similar between lean and obese women living in a region of high omega-3 intake, such as Hawaii. Methods: Fifty-five healthy, normal glucose tolerant women from Honolulu Hawaii, dichotomized based on pre-pregnancy BMI into lean (BMI <25 kg/m 2 , n = 29) and obese (BMI >30 kg/m 2 , n = 26), were recruited at scheduled term cesarean delivery. Maternal plasma DHA levels were analyzed by mass spectrometry. Expression of key genes involved in fatty acid oxidation and esterification were measured in placental tissue using qPCR. Total lipids were extracted from placental tissue via the Folch method. TNF-α concentration was measured by enzyme-linked immunosorbent assay in placental lysates. Results: DHA levels were higher in lean women compared to obese women (P = 0.02). However, DHA levels in obese women in Hawaii were eight times higher compared to obese Ohioan women (P=<0.0001). Placental lipid content and expression of key genes involved in fatty acid oxidation and esterification were similar (P > 0.05) between lean and obese women in Hawaii. Furthermore, TNF-α placental lysates were not different between lean and obese women. Conclusions: Though obese women in Hawaii have lower DHA levels compared to their lean counterparts, these levels remain over eight times as high as obese Ohioan women. These relatively high plasma omega-3 levels in obese women in Hawaii may suppress placental lipid esterification/storage and inflammation to the same levels of lean women, as seen previously in vitro.

UR - http://www.scopus.com/inward/record.url?scp=85050892635&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85050892635&partnerID=8YFLogxK

U2 - 10.1016/j.placenta.2018.07.016

DO - 10.1016/j.placenta.2018.07.016

M3 - Article

VL - 69

SP - 96

EP - 101

JO - Placenta

JF - Placenta

SN - 0143-4004

ER -