Matrix Gla protein C-terminal region binds to vitronectin. Co-localization suggests binding occurs during tissue development

Satoru Nishimoto, Miyako Nishimoto

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Matrix Gla protein (MGP) regulates calcification in cartilage and arteries. MGP synthesis during embryonic development and its binding and regulation of growth factors and morphogens of the TGF-beta/BMP superfamily suggests that it has additional functions. Assay by far-western gel overlays and gel filtration shift shows MGP binds vitronectin. Binding is saturable and consistent with a single class of binding sites. MGP binds to vitronectin but not collagen, fibromodulin, heparin, osteocalcin, chondroitin sulfate, laminin, ovalbumin or albumin. We have identified a vitronectin binding site within a 17-amino acid peptide 61-77 near the carboxyl-terminus that corresponds to a naturally occurring MGP C-terminus. MGP and the 61-77 MGP peptide also binds to fibronectin. MGP and vitronectin are focally co-localized in embryonic tissues. Co-localization in vivo suggests that the MGP and vitronectin interactions may modify cell-matrix interactions. Alternatively, vitronectin-bound MGP may have altered function for modulating BMP2 or TGF-β activity. The current study demonstrates that MGP has a novel binding activity for vitronectin, an extracellular protein that promotes cell-matrix interactions and regulates coagulation.

Original languageEnglish (US)
Pages (from-to)353-361
Number of pages9
JournalMatrix Biology
Volume24
Issue number5
DOIs
StatePublished - Aug 1 2005

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Vitronectin
Protein C
Cell Communication
matrix Gla protein
Binding Sites
Peptides
Chondroitin Sulfates
Osteocalcin
Ovalbumin
Laminin
Fibronectins
Transforming Growth Factor beta
Cartilage
Embryonic Development
Gel Chromatography
Heparin
Albumins
Intercellular Signaling Peptides and Proteins
Collagen
Arteries

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

Matrix Gla protein C-terminal region binds to vitronectin. Co-localization suggests binding occurs during tissue development. / Nishimoto, Satoru; Nishimoto, Miyako.

In: Matrix Biology, Vol. 24, No. 5, 01.08.2005, p. 353-361.

Research output: Contribution to journalArticle

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