Maturation of tumor vasculature by interferon-β disrupts the vascular niche of glioma stem cells

Regan Williams, T. L. Sims, L. Tracey, A. L. Myers, C. Y.C. Ng, H. Poppleton, A. C. Nathwani, Andrew M. Davidoff

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: The vascular niche necessary for cancer stem cell maintenance is a potential target for cancer therapy. Materials and Methods: Human glioma xenografts were treated with IFN-β delivered systemically via a liver-targeted, adeno-associated viral vector. The vascular niche was examined with immunofluorescence for glioma stem cells, endothelial cells, and perivascular cells. Results: Although IFN-β was not directly toxic to glioma stem cells in vitro, IFN-β decreased tumor size and the number of stem cells recovered in both heterotopic and orthotopic models. Treatment with IFN-β increased perivascular cells investing the tumor vasculature (6-fold) distancing stem cells from endothelial cells. Additionally, vascular smooth muscle cells co-cultured between stem cells and endothelial cells decreased stem cell recovery. Conclusion: Continuous delivery of IFN-β decreased the number of stem cells in glioma xenografts by disrupting the vascular niche through an increase in perivascular cells, which created a barrier between the glioma stem cells and the endothelial cells.

Original languageEnglish (US)
Pages (from-to)3301-3308
Number of pages8
JournalAnticancer Research
Volume30
Issue number9
StatePublished - Jan 1 2010
Externally publishedYes

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Stem Cell Niche
Glioma
Interferons
Blood Vessels
Stem Cells
Neoplasms
Endothelial Cells
Heterografts
Neoplastic Stem Cells
Poisons
Vascular Smooth Muscle
Smooth Muscle Myocytes
Fluorescent Antibody Technique
Maintenance
Liver

All Science Journal Classification (ASJC) codes

  • Oncology
  • Cancer Research

Cite this

Williams, R., Sims, T. L., Tracey, L., Myers, A. L., Ng, C. Y. C., Poppleton, H., ... Davidoff, A. M. (2010). Maturation of tumor vasculature by interferon-β disrupts the vascular niche of glioma stem cells. Anticancer Research, 30(9), 3301-3308.

Maturation of tumor vasculature by interferon-β disrupts the vascular niche of glioma stem cells. / Williams, Regan; Sims, T. L.; Tracey, L.; Myers, A. L.; Ng, C. Y.C.; Poppleton, H.; Nathwani, A. C.; Davidoff, Andrew M.

In: Anticancer Research, Vol. 30, No. 9, 01.01.2010, p. 3301-3308.

Research output: Contribution to journalArticle

Williams, R, Sims, TL, Tracey, L, Myers, AL, Ng, CYC, Poppleton, H, Nathwani, AC & Davidoff, AM 2010, 'Maturation of tumor vasculature by interferon-β disrupts the vascular niche of glioma stem cells', Anticancer Research, vol. 30, no. 9, pp. 3301-3308.
Williams R, Sims TL, Tracey L, Myers AL, Ng CYC, Poppleton H et al. Maturation of tumor vasculature by interferon-β disrupts the vascular niche of glioma stem cells. Anticancer Research. 2010 Jan 1;30(9):3301-3308.
Williams, Regan ; Sims, T. L. ; Tracey, L. ; Myers, A. L. ; Ng, C. Y.C. ; Poppleton, H. ; Nathwani, A. C. ; Davidoff, Andrew M. / Maturation of tumor vasculature by interferon-β disrupts the vascular niche of glioma stem cells. In: Anticancer Research. 2010 ; Vol. 30, No. 9. pp. 3301-3308.
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