Measurement of functional fibrinogen levels using the Thrombelastograph

Roger C. Carroll, Robert M. Craft, Jack J. Chavez, Carolyn C. Snider, Ryan K. Kirby, Eli Cohen

Research output: Contribution to journalArticle

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Abstract

Study Objective: To validate a Thromboelastograph (Haemoscope Corporation, Niles, IL) assay for functional fibrinogen. Design: Correlation study of the Thromboelastograph assay with two conventional fibrinogen assays by the standard Clauss method. Setting: Research laboratory of a university medical center. Participants and Interventions: Blood samples were obtained from 19 healthy volunteers. Measurement and Main Results: Thromboelastograph assays, using heparinized whole blood from 19 healthy donors, indicated that reptilase-XIIIa mixture (Activatorf)-generated clot shear elasticity in dynes per square centimeter (Gf) correlated with fibrinogen (mg/dL). Blood from four donors was used to define the contribution of hematocrit (Hct) to Gf by titration with platelet-rich plasma. The Gf versus Hct gave linear correlations (r2 = 0.746) with Gf = 1258 - 17.8 × % Hct. A commercial collection of 19 normal, 10 borderline, and one deficient for functional fibrinogen-citrated plasmas was assayed for Gf after recalcification using Activatorf. Of the 30 plasma samples, four were from factor X- or factor VII-deficient donors and one was from a coumadin-treated donor. There was a linear correlation of Activatorf Gf with functional fibrinogen (r2 = 0.940) with Gf = -730 + 9.21 × fibrinogen (mg/dL). Conclusion: Thrombelastography with Activatorf may be used to determine fibrinogen levels in whole blood.

Original languageEnglish (US)
Pages (from-to)186-190
Number of pages5
JournalJournal of Clinical Anesthesia
Volume20
Issue number3
DOIs
StatePublished - May 1 2008

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Fibrinogen
Hematocrit
Blood Donors
Batroxobin
Tissue Donors
Thrombelastography
Factor X
Factor VII
Platelet-Rich Plasma
Elasticity
Warfarin
Healthy Volunteers
Research

All Science Journal Classification (ASJC) codes

  • Anesthesiology and Pain Medicine

Cite this

Measurement of functional fibrinogen levels using the Thrombelastograph. / Carroll, Roger C.; Craft, Robert M.; Chavez, Jack J.; Snider, Carolyn C.; Kirby, Ryan K.; Cohen, Eli.

In: Journal of Clinical Anesthesia, Vol. 20, No. 3, 01.05.2008, p. 186-190.

Research output: Contribution to journalArticle

Carroll, Roger C. ; Craft, Robert M. ; Chavez, Jack J. ; Snider, Carolyn C. ; Kirby, Ryan K. ; Cohen, Eli. / Measurement of functional fibrinogen levels using the Thrombelastograph. In: Journal of Clinical Anesthesia. 2008 ; Vol. 20, No. 3. pp. 186-190.
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abstract = "Study Objective: To validate a Thromboelastograph (Haemoscope Corporation, Niles, IL) assay for functional fibrinogen. Design: Correlation study of the Thromboelastograph assay with two conventional fibrinogen assays by the standard Clauss method. Setting: Research laboratory of a university medical center. Participants and Interventions: Blood samples were obtained from 19 healthy volunteers. Measurement and Main Results: Thromboelastograph assays, using heparinized whole blood from 19 healthy donors, indicated that reptilase-XIIIa mixture (Activatorf)-generated clot shear elasticity in dynes per square centimeter (Gf) correlated with fibrinogen (mg/dL). Blood from four donors was used to define the contribution of hematocrit (Hct) to Gf by titration with platelet-rich plasma. The Gf versus Hct gave linear correlations (r2 = 0.746) with Gf = 1258 - 17.8 × {\%} Hct. A commercial collection of 19 normal, 10 borderline, and one deficient for functional fibrinogen-citrated plasmas was assayed for Gf after recalcification using Activatorf. Of the 30 plasma samples, four were from factor X- or factor VII-deficient donors and one was from a coumadin-treated donor. There was a linear correlation of Activatorf Gf with functional fibrinogen (r2 = 0.940) with Gf = -730 + 9.21 × fibrinogen (mg/dL). Conclusion: Thrombelastography with Activatorf may be used to determine fibrinogen levels in whole blood.",
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