Measurement of glucose turnover in cows with a low infusion of [6,6-2h2]glucose

D. L. Palmquist, J. W. Hazev, A. D. Beaulieu, C. A. Fernandez, L. Powers, Michelle Puchowicz, F. David, H. Brunengraber

Research output: Contribution to journalArticle

Abstract

Measurement of glucose turnover with [6,6-2H2]glucose requires that the isotopic enrichment (IE) of plasma glucose be at least 1 %, when using common glucose derivatives and GCMS. The cost of the tracer is prohibitive for large animal investigations. The sensitivity of the assay can be markedly increased by using a modification of the assay of single :H labeling on C6 of glucose (Landau et al ICI 95: 172-178, 1995). (6,6-2H2]Glucose reacts with NalO, to form M+2 [22]formaldehyde which is polymerized to hexamethylene tetramine (HMT). The M+2 IE of HMT is about 6 times that of glucose. Conversion of glucose to HMT is done in a single tube, without distillation of formaldehyde. The M+2 IE of HMT is easily measured above a low natural IE (0.31%). A glucose IE of 0.1 % can easily be measured because of the amplification of the M+2 IE in HMT vs glucose. We measured glucose turnover in lactating cows before and after a 500 g intragastric bolus of 1,2-propanediol (PPD, a gluconeogenic substrate). Glucose turnover (1620 yumol-muT'-kg-1) was not affected by PPD, but glucose concentration increased from 3 to 4 mM, reflecting a decrease in glucose disposal. This probably results from peripheral utilization of lactate derived from PPD.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number3
StatePublished - 1996
Externally publishedYes

Fingerprint

tetramethylenedisulfotetramine
Glucose
cows
glucose
Tuberculin
formaldehyde
Formaldehyde
Assays
propanediols
Propylene Glycol
Distillation
assays
distillation
Gas Chromatography-Mass Spectrometry
Labeling
lactates
Amplification
tracer techniques
Lactic Acid
Animals

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Palmquist, D. L., Hazev, J. W., Beaulieu, A. D., Fernandez, C. A., Powers, L., Puchowicz, M., ... Brunengraber, H. (1996). Measurement of glucose turnover in cows with a low infusion of [6,6-2h2]glucose. FASEB Journal, 10(3).

Measurement of glucose turnover in cows with a low infusion of [6,6-2h2]glucose. / Palmquist, D. L.; Hazev, J. W.; Beaulieu, A. D.; Fernandez, C. A.; Powers, L.; Puchowicz, Michelle; David, F.; Brunengraber, H.

In: FASEB Journal, Vol. 10, No. 3, 1996.

Research output: Contribution to journalArticle

Palmquist, DL, Hazev, JW, Beaulieu, AD, Fernandez, CA, Powers, L, Puchowicz, M, David, F & Brunengraber, H 1996, 'Measurement of glucose turnover in cows with a low infusion of [6,6-2h2]glucose', FASEB Journal, vol. 10, no. 3.
Palmquist DL, Hazev JW, Beaulieu AD, Fernandez CA, Powers L, Puchowicz M et al. Measurement of glucose turnover in cows with a low infusion of [6,6-2h2]glucose. FASEB Journal. 1996;10(3).
Palmquist, D. L. ; Hazev, J. W. ; Beaulieu, A. D. ; Fernandez, C. A. ; Powers, L. ; Puchowicz, Michelle ; David, F. ; Brunengraber, H. / Measurement of glucose turnover in cows with a low infusion of [6,6-2h2]glucose. In: FASEB Journal. 1996 ; Vol. 10, No. 3.
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AU - Powers, L.

AU - Puchowicz, Michelle

AU - David, F.

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