Mechanical forces alter growth factor release by pleural mesothelial cells

Christopher Waters, Julie Y. Chang, Matthew R. Glucksberg, Natacha DePaola, James B. Grotberg

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The mesothelial cells that form the visceral pleura of the lung are subjected to physical forces such as stretch due to lung expansion and fluid shear stress due to the sliding motion of the lung against the chest wall. In this study, the effect of mechanical forces on the production of growth factors by mesothelial cells was investigated. Rat visceral pleura mesothelial (RVPM) cells were exposed to fluid shear stress by perfusing a column of cell-covered beads. RVPM cells grown on a silicone elastomer were subjected to cyclic strain by applying an oscillating vacuum to the bottom of the wells using the Flexercell apparatus. Fluid shear stress (5.2-15.7 dyn/cm 2 ) stimulated the release of endothelin-1 (ET-1) by RVPM cells two- to fivefold over static cells. ET-1 secretion by RVPM cells was also stimulated approximately twofold by cyclic stretch (20% maximum strain, 30 cycles/min). RVPM cells released significant levels of platelet-derived growth factor (PDGF), but there was no effect of either shear stress or cyclic strain on PDGF release. These results suggest that the production of growth factors by pleural mesothelial cells is regulated in part by physical forces.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume272
Issue number3 16-3
StatePublished - Mar 1 1997

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Intercellular Signaling Peptides and Proteins
Pleura
Platelet-Derived Growth Factor
Endothelin-1
Lung
Silicone Elastomers
Thoracic Wall
Vacuum

All Science Journal Classification (ASJC) codes

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

Waters, C., Chang, J. Y., Glucksberg, M. R., DePaola, N., & Grotberg, J. B. (1997). Mechanical forces alter growth factor release by pleural mesothelial cells. American Journal of Physiology - Lung Cellular and Molecular Physiology, 272(3 16-3).

Mechanical forces alter growth factor release by pleural mesothelial cells. / Waters, Christopher; Chang, Julie Y.; Glucksberg, Matthew R.; DePaola, Natacha; Grotberg, James B.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 272, No. 3 16-3, 01.03.1997.

Research output: Contribution to journalArticle

Waters, C, Chang, JY, Glucksberg, MR, DePaola, N & Grotberg, JB 1997, 'Mechanical forces alter growth factor release by pleural mesothelial cells', American Journal of Physiology - Lung Cellular and Molecular Physiology, vol. 272, no. 3 16-3.
Waters, Christopher ; Chang, Julie Y. ; Glucksberg, Matthew R. ; DePaola, Natacha ; Grotberg, James B. / Mechanical forces alter growth factor release by pleural mesothelial cells. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 1997 ; Vol. 272, No. 3 16-3.
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