Mechanical perturbation of cultured cortical neurons reveals a stretch- induced delayed depolarization

Steven Tavalin, E. F. Ellis, L. S. Satin

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

1. An in vitro cellular model of injury was used to elucidate mechanisms contributing to traumatic brain injury (TBI). Neonatal rat cortical neurons cultured on a flexible silastic membrane were stretched rapidly and reversibly by a 50-ms pulse of pressurized air. 2. Sublethal cell stretch depolarized neuronal resting membrane potential by ~10 mV but only if cells were incubated for 1 h after injury. Stretch-induced delayed depolarization (or SIDD) returned to baseline values within 24 h. 3. SIDD was dependent on the degree of cell stretch and required neuronal firing, calcium entry, and N-methyl-D-aspartate receptor activation for its induction but not its maintainance. 4. Similarities between SIDD and TBI suggest that SIDD may play a role in brain injury.

Original languageEnglish (US)
Pages (from-to)2767-2773
Number of pages7
JournalJournal of Neurophysiology
Volume74
Issue number6
DOIs
StatePublished - Jan 1 1995
Externally publishedYes

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Neurons
Compressed Air
Wounds and Injuries
N-Methyl-D-Aspartate Receptors
Membrane Potentials
Brain Injuries
Calcium
Membranes
Traumatic Brain Injury
baysilon
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Physiology

Cite this

Mechanical perturbation of cultured cortical neurons reveals a stretch- induced delayed depolarization. / Tavalin, Steven; Ellis, E. F.; Satin, L. S.

In: Journal of Neurophysiology, Vol. 74, No. 6, 01.01.1995, p. 2767-2773.

Research output: Contribution to journalArticle

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