Mechanical stretching of alveolar epithelial cells increases Na+-K+- ATPase activity

Christopher Waters, Karen M. Ridge, G. Sunio, K. Venetsanou, Jacob Iasha Sznajder

Research output: Contribution to journalArticle

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Abstract

Alveolar epithelial cells effect edema clearance by transporting Na+ and liquid out of the air spaces. Active Na+ transport by the basolaterally located Na+-K+-ATPase is an important contributor to lung edema clearance. Because alveoli undergo cyclic stretch in vivo, we investigated the role of cyclic stretch in the regulation of Na+-K+-ATPase activity in alveolar epithelial cells. Using the Flexercell Strain Unit, we exposed a cell line of murine lung epithelial cells (MLE-12) to cyclic stretch (30 cycles/min). After 15 min of stretch (10% mean strain), there was no change in Na+-K+- ATPase activity, as assessed by 86Rb+ uptake. By 30 min and after 60 min, Na+-K+-ATPase activity was significantly increased. When cells were treated with amiloride to block amiloride-sensitive Na+ entry into cells or when cells were treated with gadolinium to block stretch-activated, nonselective cation channels, there was no stimulation of Na+-K+-ATPase activity by cyclic stretch. Conversely, cells exposed to Nystatin, which increases Na+ entry into cells, demonstrated increased Na+K+ATPase activity. The changes in Na+-K+-ATPase activity were paralleled by increased Na+-K+-ATPase protein in the basolateral membrane of MLE-12 cells. Thus, in MLE-12 cells, short-term cyclic stretch stimulates Na+-K+-ATPase activity, most likely by increasing intracellular Na+ and by recruitment of Na+-K+-ATPase subunits from intracellular pools to the basolateral membrane.

Original languageEnglish (US)
Pages (from-to)715-721
Number of pages7
JournalJournal of Applied Physiology
Volume87
Issue number2
StatePublished - Aug 18 1999

Fingerprint

Alveolar Epithelial Cells
Amiloride
Edema
sodium-translocating ATPase
Nystatin
Lung
Membranes
Active Biological Transport
Gadolinium
Cations
Epithelial Cells
Air

All Science Journal Classification (ASJC) codes

  • Physiology
  • Physiology (medical)

Cite this

Waters, C., Ridge, K. M., Sunio, G., Venetsanou, K., & Sznajder, J. I. (1999). Mechanical stretching of alveolar epithelial cells increases Na+-K+- ATPase activity. Journal of Applied Physiology, 87(2), 715-721.

Mechanical stretching of alveolar epithelial cells increases Na+-K+- ATPase activity. / Waters, Christopher; Ridge, Karen M.; Sunio, G.; Venetsanou, K.; Sznajder, Jacob Iasha.

In: Journal of Applied Physiology, Vol. 87, No. 2, 18.08.1999, p. 715-721.

Research output: Contribution to journalArticle

Waters, C, Ridge, KM, Sunio, G, Venetsanou, K & Sznajder, JI 1999, 'Mechanical stretching of alveolar epithelial cells increases Na+-K+- ATPase activity', Journal of Applied Physiology, vol. 87, no. 2, pp. 715-721.
Waters C, Ridge KM, Sunio G, Venetsanou K, Sznajder JI. Mechanical stretching of alveolar epithelial cells increases Na+-K+- ATPase activity. Journal of Applied Physiology. 1999 Aug 18;87(2):715-721.
Waters, Christopher ; Ridge, Karen M. ; Sunio, G. ; Venetsanou, K. ; Sznajder, Jacob Iasha. / Mechanical stretching of alveolar epithelial cells increases Na+-K+- ATPase activity. In: Journal of Applied Physiology. 1999 ; Vol. 87, No. 2. pp. 715-721.
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