Mechanisms of the oxytocic activity of papaya proteinases

P. Ganesan Adaikan, Adebowale Adebiyi

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Crude papaya latex (CPL) and its proteinases, papain (PPN) and chymopapain (CPN), are strong uterine contractants. The current study was carried out to examine possible mechanisms of the uterine stimulating activity of the proteinases. Inactivation of the enzymatic activity of papaya proteinases reversibly abolished their uterine-stimulating effect, suggesting that enzymatic activity of the proteinases is a prerequisite for their oxytocic activity. Moreover, removal of Ca2+ from the uterine bathing medium reversibly abolished the uterine-stimulating effect of the proteinases. Nifedipine and verapamil (Ca2+ channel blockers) significantly and reversibly block CPL-, CPN-, and PPN-induced uterine contractions. Blockade of 5-hydroxytryptamine receptors did not prevent the oxytocic activity of papaya proteinases. However, uterine contractions induced by the proteinases were significantly and reversibly inhibited by meclofenamic acid (a cyclooxy-genase and prostaglandin inhibitor). At 0.3 and 1 mg/ml, CPL, CPN, and PPN caused a concentration-dependent increase in prostaglandin F, production in cultured rat uterus, but only CPL (1 mg/ml) induced PGF production by the cultured rat uterine tissues was statistically significant. The results of the current study suggest that prostaglandin release by Ca2+ mobilization- and proteolysis-dependent activity of papaya latex and its proteinases could play a major role in their oxytocic activity.

Original languageEnglish (US)
Pages (from-to)646-655
Number of pages10
JournalPharmaceutical Biology
Volume42
Issue number8
DOIs
StatePublished - Dec 1 2004
Externally publishedYes

Fingerprint

Oxytocics
Carica
Peptide Hydrolases
Latex
Chymopapain
Papain
Uterine Contraction
Meclofenamic Acid
Prostaglandin Antagonists
Dinoprost
Serotonin Receptors
Prostaglandins F
Nifedipine
Verapamil
Proteolysis
Uterus
Prostaglandins

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine

Cite this

Mechanisms of the oxytocic activity of papaya proteinases. / Adaikan, P. Ganesan; Adebiyi, Adebowale.

In: Pharmaceutical Biology, Vol. 42, No. 8, 01.12.2004, p. 646-655.

Research output: Contribution to journalArticle

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