Melatonin and its metabolites accumulate in the human epidermis in vivo and inhibit proliferation and tyrosinase activity in epidermal melanocytes in vitro

Tae Kang Kim, Zongtao Lin, William J. Tidwell, Wei Li, Andrzej T. Slominski

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Melatonin and its metabolites including 6-hydroxymelatonin (6(OH)M), N1-acetyl-N2-formyl-5-methoxykynuramine (AFMK) and 5-methoxytryptamine (5MT) are endogenously produced in human epidermis. This production depends on race, gender and age. The highest melatonin levels are in African-Americans. In each racial group they are highest in young African-Americans [30-50 years old (yo)], old Caucasians (60-90 yo) and Caucasian females. AFMK levels are the highest in African-Americans, while 6(OH)M and 5MT levels are similar in all groups. Testing of their phenotypic effects in normal human melanocytes show that melatonin and its metabolites (10-5M) inhibit tyrosinase activity and cell growth, and inhibit DNA synthesis in a dose dependent manner with 10-9M being the lowest effective concentration. In melanoma cells, they inhibited cell growth but had no effect on melanogenesis, except for 5MT which enhanced L-tyrosine induced melanogenesis. In conclusion, melatonin and its metabolites [6(OH)M, AFMK and 5MT] are produced endogenously in human epidermis and can affect melanocyte and melanoma behavior.

Original languageEnglish (US)
Pages (from-to)1-8
Number of pages8
JournalMolecular and Cellular Endocrinology
Volume404
DOIs
StatePublished - Mar 5 2015

Fingerprint

5-Methoxytryptamine
Monophenol Monooxygenase
Melanocytes
Melatonin
Metabolites
Epidermis
African Americans
Cell growth
Melanoma
Growth
Tyrosine
In Vitro Techniques
DNA
Testing

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Endocrinology

Cite this

Melatonin and its metabolites accumulate in the human epidermis in vivo and inhibit proliferation and tyrosinase activity in epidermal melanocytes in vitro. / Kim, Tae Kang; Lin, Zongtao; Tidwell, William J.; Li, Wei; Slominski, Andrzej T.

In: Molecular and Cellular Endocrinology, Vol. 404, 05.03.2015, p. 1-8.

Research output: Contribution to journalArticle

@article{8ad170995b074685a5a8c22371bb7906,
title = "Melatonin and its metabolites accumulate in the human epidermis in vivo and inhibit proliferation and tyrosinase activity in epidermal melanocytes in vitro",
abstract = "Melatonin and its metabolites including 6-hydroxymelatonin (6(OH)M), N1-acetyl-N2-formyl-5-methoxykynuramine (AFMK) and 5-methoxytryptamine (5MT) are endogenously produced in human epidermis. This production depends on race, gender and age. The highest melatonin levels are in African-Americans. In each racial group they are highest in young African-Americans [30-50 years old (yo)], old Caucasians (60-90 yo) and Caucasian females. AFMK levels are the highest in African-Americans, while 6(OH)M and 5MT levels are similar in all groups. Testing of their phenotypic effects in normal human melanocytes show that melatonin and its metabolites (10-5M) inhibit tyrosinase activity and cell growth, and inhibit DNA synthesis in a dose dependent manner with 10-9M being the lowest effective concentration. In melanoma cells, they inhibited cell growth but had no effect on melanogenesis, except for 5MT which enhanced L-tyrosine induced melanogenesis. In conclusion, melatonin and its metabolites [6(OH)M, AFMK and 5MT] are produced endogenously in human epidermis and can affect melanocyte and melanoma behavior.",
author = "Kim, {Tae Kang} and Zongtao Lin and Tidwell, {William J.} and Wei Li and Slominski, {Andrzej T.}",
year = "2015",
month = "3",
day = "5",
doi = "10.1016/j.mce.2014.07.024",
language = "English (US)",
volume = "404",
pages = "1--8",
journal = "Molecular and Cellular Endocrinology",
issn = "0303-7207",
publisher = "Elsevier Ireland Ltd",

}

TY - JOUR

T1 - Melatonin and its metabolites accumulate in the human epidermis in vivo and inhibit proliferation and tyrosinase activity in epidermal melanocytes in vitro

AU - Kim, Tae Kang

AU - Lin, Zongtao

AU - Tidwell, William J.

AU - Li, Wei

AU - Slominski, Andrzej T.

PY - 2015/3/5

Y1 - 2015/3/5

N2 - Melatonin and its metabolites including 6-hydroxymelatonin (6(OH)M), N1-acetyl-N2-formyl-5-methoxykynuramine (AFMK) and 5-methoxytryptamine (5MT) are endogenously produced in human epidermis. This production depends on race, gender and age. The highest melatonin levels are in African-Americans. In each racial group they are highest in young African-Americans [30-50 years old (yo)], old Caucasians (60-90 yo) and Caucasian females. AFMK levels are the highest in African-Americans, while 6(OH)M and 5MT levels are similar in all groups. Testing of their phenotypic effects in normal human melanocytes show that melatonin and its metabolites (10-5M) inhibit tyrosinase activity and cell growth, and inhibit DNA synthesis in a dose dependent manner with 10-9M being the lowest effective concentration. In melanoma cells, they inhibited cell growth but had no effect on melanogenesis, except for 5MT which enhanced L-tyrosine induced melanogenesis. In conclusion, melatonin and its metabolites [6(OH)M, AFMK and 5MT] are produced endogenously in human epidermis and can affect melanocyte and melanoma behavior.

AB - Melatonin and its metabolites including 6-hydroxymelatonin (6(OH)M), N1-acetyl-N2-formyl-5-methoxykynuramine (AFMK) and 5-methoxytryptamine (5MT) are endogenously produced in human epidermis. This production depends on race, gender and age. The highest melatonin levels are in African-Americans. In each racial group they are highest in young African-Americans [30-50 years old (yo)], old Caucasians (60-90 yo) and Caucasian females. AFMK levels are the highest in African-Americans, while 6(OH)M and 5MT levels are similar in all groups. Testing of their phenotypic effects in normal human melanocytes show that melatonin and its metabolites (10-5M) inhibit tyrosinase activity and cell growth, and inhibit DNA synthesis in a dose dependent manner with 10-9M being the lowest effective concentration. In melanoma cells, they inhibited cell growth but had no effect on melanogenesis, except for 5MT which enhanced L-tyrosine induced melanogenesis. In conclusion, melatonin and its metabolites [6(OH)M, AFMK and 5MT] are produced endogenously in human epidermis and can affect melanocyte and melanoma behavior.

UR - http://www.scopus.com/inward/record.url?scp=84922273922&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84922273922&partnerID=8YFLogxK

U2 - 10.1016/j.mce.2014.07.024

DO - 10.1016/j.mce.2014.07.024

M3 - Article

VL - 404

SP - 1

EP - 8

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

SN - 0303-7207

ER -