Metabolic regulation of gene transcription

A. L. Gurney, Edwards Park, J. Liu, M. Giralt, M. M. McGrane, Y. M. Patel, D. R. Crawford, S. E. Nizielski, S. Savon, R. W. Hanson

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The impact of nutrients on gene expression has become an area of considerable interest as the number of genes coding for key regulatory proteins in metabolic pathways are studied in detail. This has been greatly aided by a number of new techniques developed to study gene transcription in animals. We will use as an example studies on the regulation of transcription of the gene coding for P-enolpyruvate carboxykinase, a key enzyme in hepatic and renal gluconeogenesis. The promoter for P-enolpyruvate carboxykinase contains a number of regulatory elements within 500 bp of the start-site of gene transcription that are required for the response of the gene to metabolic signals. These elements bind tissue-specific transcription factors in complex patterns of interactions, which result in the coordinate control of P-enolpyruvate carboxykinase gene expression. An analysis of the regulation of transcription of this gene involves the use of a number of techniques ranging from gene transfection into cells in culture to the introduction of chimeric genes containing the P-enolpyruvate carboxykinase promoter into transgenic mice. This review presents a progress report on the current status of research on the nutritional and hormonal regulation of transcription of the P-enolpyruvate carboxykinase gene.

Original languageEnglish (US)
JournalJournal of Nutrition
Volume124
Issue number8 SUPPL.
StatePublished - 1994
Externally publishedYes

Fingerprint

transcription (genetics)
Genes
genes
promoter regions
Gene Expression
gene expression
Gluconeogenesis
gluconeogenesis
Transcription Initiation Site
hormonal regulation
regulatory proteins
transfection
Metabolic Networks and Pathways
Transgenic Mice
Transfection
biochemical pathways
cell culture
Transcription Factors
transcription factors
Cell Culture Techniques

All Science Journal Classification (ASJC) codes

  • Food Science
  • Medicine (miscellaneous)

Cite this

Gurney, A. L., Park, E., Liu, J., Giralt, M., McGrane, M. M., Patel, Y. M., ... Hanson, R. W. (1994). Metabolic regulation of gene transcription. Journal of Nutrition, 124(8 SUPPL.).

Metabolic regulation of gene transcription. / Gurney, A. L.; Park, Edwards; Liu, J.; Giralt, M.; McGrane, M. M.; Patel, Y. M.; Crawford, D. R.; Nizielski, S. E.; Savon, S.; Hanson, R. W.

In: Journal of Nutrition, Vol. 124, No. 8 SUPPL., 1994.

Research output: Contribution to journalArticle

Gurney, AL, Park, E, Liu, J, Giralt, M, McGrane, MM, Patel, YM, Crawford, DR, Nizielski, SE, Savon, S & Hanson, RW 1994, 'Metabolic regulation of gene transcription', Journal of Nutrition, vol. 124, no. 8 SUPPL..
Gurney AL, Park E, Liu J, Giralt M, McGrane MM, Patel YM et al. Metabolic regulation of gene transcription. Journal of Nutrition. 1994;124(8 SUPPL.).
Gurney, A. L. ; Park, Edwards ; Liu, J. ; Giralt, M. ; McGrane, M. M. ; Patel, Y. M. ; Crawford, D. R. ; Nizielski, S. E. ; Savon, S. ; Hanson, R. W. / Metabolic regulation of gene transcription. In: Journal of Nutrition. 1994 ; Vol. 124, No. 8 SUPPL.
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