Metabolism of [14C] arachidonic acid in the isolated rabbit spleen

Toshihiro Hidaka, Patrick Y.K. Wong, Kafait Malik

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Infusion of [14C] arachidonic acid (AA) into the isolated, Tyrode perfused rabbit spleen resulted in the release of a substance into the venous effluent with the musculotropic activity and chromatographic properties of prostaglandin (PG)E2. Smaller amounts of radioactive materials with the chromatographic properties of PGF, 6-keto-PGF, and PGD2 were also released. The radiolabeled material released in largest amounts from the spleen was identified as PGE2 on the basis of: 1) Co-chromatography with PGE2 in three solvent systems, 2) Conversion of the radioactive material and of authentic [3H] PGE2 to similar products by treatment with sodium borohydride and with potassium hydroxide, and 3) Stability of the musculotropic activity in Tyrode solution at 37°C. Release of the major and minor radioactive products was inhibited by pretreatment of the spleen with either indomethacin or 5,8,11,14-eicosatetraynoic acid.

Original languageEnglish (US)
Pages (from-to)805-818
Number of pages14
JournalProstaglandins
Volume19
Issue number5
DOIs
StatePublished - Jan 1 1980

Fingerprint

Dinoprostone
Arachidonic Acid
Metabolism
Spleen
Rabbits
Radioactive materials
5,8,11,14-Eicosatetraynoic Acid
Prostaglandin D2
Dinoprost
Chromatography
Indomethacin
Effluents

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Endocrinology

Cite this

Metabolism of [14C] arachidonic acid in the isolated rabbit spleen. / Hidaka, Toshihiro; Wong, Patrick Y.K.; Malik, Kafait.

In: Prostaglandins, Vol. 19, No. 5, 01.01.1980, p. 805-818.

Research output: Contribution to journalArticle

Hidaka, Toshihiro ; Wong, Patrick Y.K. ; Malik, Kafait. / Metabolism of [14C] arachidonic acid in the isolated rabbit spleen. In: Prostaglandins. 1980 ; Vol. 19, No. 5. pp. 805-818.
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