Metallothionein inhibits peroxynitrite-induced DNA and lipoprotein damage

Lu Cai, Jon B. Klein, Yujian Kang

Research output: Contribution to journalArticle

129 Citations (Scopus)

Abstract

Previous studies have demonstrated that metallothionein functions as an antioxidant that protects against oxidative DNA, protein, and lipid damage induced by superoxide anion, hydrogen peroxide, hydroxyl radical, and nitric oxide. The present study was undertaken to test the hypothesis that metallothionein also protects from DNA and lipoprotein damage induced by peroxynitrite, an important reactive nitrogen species that causes a diversity of pathological processes. A cell-free system was used. DNA damage was detected by the mobility of plasmid DNA in electrophoresis. Oxidation of low density lipoprotein was measured by a thiobarbituric acid-reactive substance, which was confirmed by lipid hydroperoxide assay. Plasmid DNA damage and low density lipoprotein oxidation were induced by 3-morpholinosydnomine, which produces peroxynitrite through the reaction between nitric oxide and superoxide anion or by synthesized peroxynitrite directly. DNA damage by 3-morpholinosydnomine was prevented by both metallothionein and superoxide dismutase, whereas the damage caused by peroxynitrite was prevented by metallothionein only. The oxidation of low density lipoprotein by 3-morpholinosydnomine and peroxynitrite was also significantly inhibited by metallothionein. This study thus demonstrates that metallothionein may react directly with peroxynitrite to prevent DNA and lipoprotein damage induced by this pathological reactive nitrogen species.

Original languageEnglish (US)
Pages (from-to)38957-38960
Number of pages4
JournalJournal of Biological Chemistry
Volume275
Issue number50
DOIs
StatePublished - Dec 15 2000

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Peroxynitrous Acid
Metallothionein
Lipoproteins
DNA Damage
DNA
LDL Lipoproteins
Reactive Nitrogen Species
Superoxides
Nitric Oxide
Oxidation
Plasmids
Thiobarbituric Acid Reactive Substances
Cell-Free System
Lipid Peroxides
Pathologic Processes
Hydroxyl Radical
Hydrogen Peroxide
Superoxide Dismutase
Electrophoresis
Antioxidants

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Metallothionein inhibits peroxynitrite-induced DNA and lipoprotein damage. / Cai, Lu; Klein, Jon B.; Kang, Yujian.

In: Journal of Biological Chemistry, Vol. 275, No. 50, 15.12.2000, p. 38957-38960.

Research output: Contribution to journalArticle

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