Methionine enkephalin-like, substance P-like, and β-endorphin-like immunoreactivity in human parotid saliva

D. L. Pikula, Edward Harris, D. M. Desiderio, G. H. Fridland, J. L. Lovelace

Research output: Contribution to journalArticle

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Abstract

These three neuropeptides were measured at daily baseline values by radioimmunoassay. Stimulated parotid saliva was collected from 31 subjects using a modified Carlson-Crittenden device affixed over Stenson's duct. Methionine enkephalin-like immunoreactivity ranged from 6.6 to 11.7 fmol/ml, with a mean of 9.3 fmol/ml. Substance P-like immunoreactivity ranged from 6.1 to 12.6 fmol/ml, with a mean of 9.3 fmol/ml. β-Endorphin-like immunoreactivity ranged from 1.2 to 3.6 fmol/ml, with a mean of 2.6 fmol/ml. This is believed to be the first documentation of methionine enkephelin- and substance P-like activities in human parotid saliva and the first demonstration of β-endorphin-like activity in any type of human saliva. Substance P-like activity was significantly higher in morning than evening samples; β-endorphin-like activity also tended to be higher in the morning samples. Substance P and β-endorphinlike immunoreactivities covaried in a significant positive manner, suggesting either common control mechanisms or similar responses to physiological variables.

Original languageEnglish (US)
Pages (from-to)705-709
Number of pages5
JournalArchives of Oral Biology
Volume37
Issue number9
DOIs
StatePublished - Jan 1 1992

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Endorphins
Substance P
Saliva
Methionine Enkephalin
Neuropeptides
Human Activities
Documentation
Methionine
Radioimmunoassay
Equipment and Supplies
Like substances enkephalin-Met

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Cite this

Methionine enkephalin-like, substance P-like, and β-endorphin-like immunoreactivity in human parotid saliva. / Pikula, D. L.; Harris, Edward; Desiderio, D. M.; Fridland, G. H.; Lovelace, J. L.

In: Archives of Oral Biology, Vol. 37, No. 9, 01.01.1992, p. 705-709.

Research output: Contribution to journalArticle

Pikula, D. L. ; Harris, Edward ; Desiderio, D. M. ; Fridland, G. H. ; Lovelace, J. L. / Methionine enkephalin-like, substance P-like, and β-endorphin-like immunoreactivity in human parotid saliva. In: Archives of Oral Biology. 1992 ; Vol. 37, No. 9. pp. 705-709.
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