Methylation of the enhancer region of avian sarcoma virus long terminal repeat suppresses transcription

Ramareddy Guntaka, Siddareme Gowda, Herbert Wagner, Dietrich Simon

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The effect of methylation of an enhancer on transcription was studied. A 245 bp enhancer-containing a fragment of the LTR of the avian sarcoma virus was methylated in vitro and ligated back into a vector which lacked the upstream enhancer sequence. The transient expression in QT6 cells indicated that methylation of the enhancer-containing sequence severely reduced the extent of transcription.

Original languageEnglish (US)
Pages (from-to)332-336
Number of pages5
JournalFEBS Letters
Volume221
Issue number2
DOIs
StatePublished - Sep 14 1987

Fingerprint

Avian Sarcoma Viruses
Methylation
Terminal Repeat Sequences
Transcription
Viruses

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Methylation of the enhancer region of avian sarcoma virus long terminal repeat suppresses transcription. / Guntaka, Ramareddy; Gowda, Siddareme; Wagner, Herbert; Simon, Dietrich.

In: FEBS Letters, Vol. 221, No. 2, 14.09.1987, p. 332-336.

Research output: Contribution to journalArticle

Guntaka, Ramareddy ; Gowda, Siddareme ; Wagner, Herbert ; Simon, Dietrich. / Methylation of the enhancer region of avian sarcoma virus long terminal repeat suppresses transcription. In: FEBS Letters. 1987 ; Vol. 221, No. 2. pp. 332-336.
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