Microinjection into Xenopus oocytes

a precise semi-automatic instrument and optimal parameters for injection of mRNAs

Gabor Tigyi, Ian Parker

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A new apparatus for the injection of Xenopus oocytes is described which provides semi-automatic cell handling together with highly accurate and reproducible volume delivery. Using the system requires very little skill, yet it gives 6.3% average reproducibility in the 5 to 70 nl volume range. The instrument uses a fixed injector system driven by an Inchworm piezoelectric positioner or, in a low-cost versio, by a Rainin EDP-2 battery-operated motorized pipetted. A movable, vacuum-operated oocyte holder minimizes laterla movement of the oocyte during injection. Oocytes injected with the system show better survival and enhanced expression of mRNA compared with those injected with a widely used type of manual injector (Coleman, 1984).

Original languageEnglish (US)
Pages (from-to)243-252
Number of pages10
JournalJournal of Biochemical and Biophysical Methods
Volume22
Issue number3
DOIs
StatePublished - Jan 1 1991

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Microinjections
Xenopus
Oocytes
Vacuum
Messenger RNA
Injections
Costs
Costs and Cost Analysis

All Science Journal Classification (ASJC) codes

  • Biophysics

Cite this

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abstract = "A new apparatus for the injection of Xenopus oocytes is described which provides semi-automatic cell handling together with highly accurate and reproducible volume delivery. Using the system requires very little skill, yet it gives 6.3{\%} average reproducibility in the 5 to 70 nl volume range. The instrument uses a fixed injector system driven by an Inchworm piezoelectric positioner or, in a low-cost versio, by a Rainin EDP-2 battery-operated motorized pipetted. A movable, vacuum-operated oocyte holder minimizes laterla movement of the oocyte during injection. Oocytes injected with the system show better survival and enhanced expression of mRNA compared with those injected with a widely used type of manual injector (Coleman, 1984).",
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