Microphthalmia transcription factor: A sensitive and specific melanocyte marker for melanoma diagnosis

Roy King, Katherine N. Weilbaecher, Gaël McGill, Edward Cooley, Martin Mihm, David E. Fisher

Research output: Contribution to journalArticle

204 Citations (Scopus)

Abstract

Malignant melanomas do not uniformly retain expression of melanocytic gene products - an observation associated with diagnostic dilemmas. Microphthalmia transcription factor (Mitf) is a melanocytic nuclear protein critical for the embryonic development and postnatal viability of melanocytes. It serves as a master regulator in modulating extracellular signals, such as those triggered by α-MSH and c-Kit ligand. Because of its central role in melanocyte survival and to assess its potential use as a histopathological marker for melanoma, Mitf expression was examined in histologically confirmed human melanoma specimens. Western blot analysis of melanoma cell lines revealed consistent expression of two Mitf protein isoforms differing by MAP kinase-mediated phosphorylation. In a series of 76 consecutive human melanoma surgical specimens, 100% stained positively for Mitf with a nuclear pattern of reactivity. In a side-by-side comparison, Mitf staining was positive in melanomas that failed to stain for either HMB-45 or S-100, the most common currently used melanoma markers. Of 60 non-melanoma tumors, none displayed nuclear Mitf staining and two displayed cytoplasmic staining. Although Mitf does not distinguish benign from malignant melanocytic lesions, for invasive neoplasms it appears to be a highly sensitive and specific histopathological melanocyte marker for melanoma.

Original languageEnglish (US)
Pages (from-to)731-738
Number of pages8
JournalAmerican Journal of Pathology
Volume155
Issue number3
DOIs
StatePublished - Jan 1 1999

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Microphthalmia-Associated Transcription Factor
Melanocytes
Melanoma
Staining and Labeling
Melanocyte-Stimulating Hormones
Stem Cell Factor
Nuclear Proteins
Embryonic Development
Neoplasms
Protein Isoforms
Coloring Agents
Phosphotransferases
Western Blotting
Phosphorylation
Observation
Gene Expression
Cell Line

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

Microphthalmia transcription factor : A sensitive and specific melanocyte marker for melanoma diagnosis. / King, Roy; Weilbaecher, Katherine N.; McGill, Gaël; Cooley, Edward; Mihm, Martin; Fisher, David E.

In: American Journal of Pathology, Vol. 155, No. 3, 01.01.1999, p. 731-738.

Research output: Contribution to journalArticle

King, Roy ; Weilbaecher, Katherine N. ; McGill, Gaël ; Cooley, Edward ; Mihm, Martin ; Fisher, David E. / Microphthalmia transcription factor : A sensitive and specific melanocyte marker for melanoma diagnosis. In: American Journal of Pathology. 1999 ; Vol. 155, No. 3. pp. 731-738.
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