MicroRNA Profiling in Prostate Cancer - The Diagnostic Potential of Urinary miR-205 and miR-214

Anvesha Srivastava, Helle Goldberger, Alexander Dimtchev, Malathi Ramalinga, Juliet Chijioke, Catalin Marian, Eric K. Oermann, Sunghae Uhm, Joy S. Kim, Leonard N. Chen, Xin Li, Deborah L. Berry, Bhaskar V.S. Kallakury, Subhash Chauhan, Sean P. Collins, Simeng Suy, Deepak Kumar

Research output: Contribution to journalArticle

103 Citations (Scopus)

Abstract

Prostate cancer (PCa) is the most common type of cancer in men in the United States, which disproportionately affects African American descents. While metastasis is the most common cause of death among PCa patients, no specific markers have been assigned to severity and ethnic biasness of the disease. MicroRNAs represent a promising new class of biomarkers owing to their inherent stability and resilience. In the present study, we investigated potential miRNAs that can be used as biomarkers and/or therapeutic targets and can provide insight into the severity and ethnic biasness of PCa. PCR array was performed in FFPE PCa tissues (5 Caucasian American and 5 African American) and selected differentially expressed miRNAs were validated by qRT-PCR, in 40 (15 CA and 25 AA) paired PCa and adjacent normal tissues. Significantly deregulated miRNAs were also analyzed in urine samples to explore their potential as non-invasive biomarker for PCa. Out of 8 miRNAs selected for validation from PCR array data, miR-205 (p<0.0001), mir-214 (p<0.0001), miR-221(p<0.001) and miR-99b (p<0.0001) were significantly downregulated in PCa tissues. ROC curve shows that all four miRNAs successfully discriminated between PCa and adjacent normal tissues. MiR-99b showed significant down regulation (p<0.01) in AA PCa tissues as compared to CA PCa tissues and might be related to the aggressiveness associated with AA population. In urine, miR-205 (p<0.05) and miR-214 (p<0.05) were significantly downregulated in PCa patients and can discriminate PCa patients from healthy individuals with 89% sensitivity and 80% specificity. In conclusion, present study showed that miR-205 and miR-214 are downregulated in PCa and may serve as potential non-invasive molecular biomarker for PCa.

Original languageEnglish (US)
Article numbere76994
JournalPloS one
Volume8
Issue number10
DOIs
StatePublished - Oct 22 2013

Fingerprint

prostatic neoplasms
MicroRNAs
microRNA
Prostatic Neoplasms
Biomarkers
Tissue
biomarkers
Down-Regulation
African Americans
Polymerase Chain Reaction
urine
Urine
ROC Curve
metastasis
tissues
aggression
Cause of Death

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Srivastava, A., Goldberger, H., Dimtchev, A., Ramalinga, M., Chijioke, J., Marian, C., ... Kumar, D. (2013). MicroRNA Profiling in Prostate Cancer - The Diagnostic Potential of Urinary miR-205 and miR-214. PloS one, 8(10), [e76994]. https://doi.org/10.1371/journal.pone.0076994

MicroRNA Profiling in Prostate Cancer - The Diagnostic Potential of Urinary miR-205 and miR-214. / Srivastava, Anvesha; Goldberger, Helle; Dimtchev, Alexander; Ramalinga, Malathi; Chijioke, Juliet; Marian, Catalin; Oermann, Eric K.; Uhm, Sunghae; Kim, Joy S.; Chen, Leonard N.; Li, Xin; Berry, Deborah L.; Kallakury, Bhaskar V.S.; Chauhan, Subhash; Collins, Sean P.; Suy, Simeng; Kumar, Deepak.

In: PloS one, Vol. 8, No. 10, e76994, 22.10.2013.

Research output: Contribution to journalArticle

Srivastava, A, Goldberger, H, Dimtchev, A, Ramalinga, M, Chijioke, J, Marian, C, Oermann, EK, Uhm, S, Kim, JS, Chen, LN, Li, X, Berry, DL, Kallakury, BVS, Chauhan, S, Collins, SP, Suy, S & Kumar, D 2013, 'MicroRNA Profiling in Prostate Cancer - The Diagnostic Potential of Urinary miR-205 and miR-214', PloS one, vol. 8, no. 10, e76994. https://doi.org/10.1371/journal.pone.0076994
Srivastava A, Goldberger H, Dimtchev A, Ramalinga M, Chijioke J, Marian C et al. MicroRNA Profiling in Prostate Cancer - The Diagnostic Potential of Urinary miR-205 and miR-214. PloS one. 2013 Oct 22;8(10). e76994. https://doi.org/10.1371/journal.pone.0076994
Srivastava, Anvesha ; Goldberger, Helle ; Dimtchev, Alexander ; Ramalinga, Malathi ; Chijioke, Juliet ; Marian, Catalin ; Oermann, Eric K. ; Uhm, Sunghae ; Kim, Joy S. ; Chen, Leonard N. ; Li, Xin ; Berry, Deborah L. ; Kallakury, Bhaskar V.S. ; Chauhan, Subhash ; Collins, Sean P. ; Suy, Simeng ; Kumar, Deepak. / MicroRNA Profiling in Prostate Cancer - The Diagnostic Potential of Urinary miR-205 and miR-214. In: PloS one. 2013 ; Vol. 8, No. 10.
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