MiR-125b Reduces Porcine Reproductive and Respiratory Syndrome Virus Replication by Negatively Regulating the NF-κB Pathway

Dang Wang, Lu Cao, Zheng Xu, Liurong Fang, Yao Zhong, Quangang Chen, Rui Luo, Huanchun Chen, Kui Li, Shaobo Xiao

Research output: Contribution to journalArticle

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Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is an Arterivirus that has been devastating the swine industry worldwide since the late 1980s. To investigate the impact of cellular microRNAs (miRNAs) on the replication of PRRSV, we screened 10 highly conserved miRNAs implicated in innate immunity or antiviral function and identified miR-125b as an inhibitor of PRRSV replication. Virus titer and western blot assays demonstrated that miR-125b reduced PRRSV replication and viral gene expression in a dose-dependent manner in both MARC-145 cell line and primary porcine alveolar macrophages. Mechanistically, miR-125b did not target the PRRSV genome. Rather, it inhibited activation of NF-κB, which we found to be required for PRRSV replication. PRRSV, in turn, down-regulated miR-125b expression post-infection to promote viral replication. Collectively, miR-125b is an antiviral host factor against PRRSV, but it is subject to manipulation by PRRSV. Our study reveals an example of manipulation of a cellular miRNA by an arterivirus to re-orchestrate host gene expression for viral propagation and sheds new light on targeting host factors to develop effective control measures for PRRS.

Original languageEnglish (US)
Article numbere55838
JournalPLoS ONE
Volume8
Issue number2
DOIs
StatePublished - Feb 7 2013

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Porcine respiratory and reproductive syndrome virus
Porcine reproductive and respiratory syndrome virus
Virus Replication
virus replication
Viruses
Arterivirus
MicroRNAs
microRNA
Antiviral Agents
Gene expression
Swine
Porcine Reproductive and Respiratory Syndrome
pork industry
Gene Expression
gene expression
Viral Genes
Alveolar Macrophages
viral load
Viral Load
Innate Immunity

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

MiR-125b Reduces Porcine Reproductive and Respiratory Syndrome Virus Replication by Negatively Regulating the NF-κB Pathway. / Wang, Dang; Cao, Lu; Xu, Zheng; Fang, Liurong; Zhong, Yao; Chen, Quangang; Luo, Rui; Chen, Huanchun; Li, Kui; Xiao, Shaobo.

In: PLoS ONE, Vol. 8, No. 2, e55838, 07.02.2013.

Research output: Contribution to journalArticle

Wang, Dang ; Cao, Lu ; Xu, Zheng ; Fang, Liurong ; Zhong, Yao ; Chen, Quangang ; Luo, Rui ; Chen, Huanchun ; Li, Kui ; Xiao, Shaobo. / MiR-125b Reduces Porcine Reproductive and Respiratory Syndrome Virus Replication by Negatively Regulating the NF-κB Pathway. In: PLoS ONE. 2013 ; Vol. 8, No. 2.
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