Model combustion-generated particulate matter containing persistent free radicals redox cycle to produce reactive oxygen species

Matthew A. Kelley, Valeria Y. Hebert, Taylor M. Thibeaux, Mackenzie A. Orchard, Farhana Hasan, Stephania A. Cormier, Paul T. Thevenot, Slawomir M. Lomnicki, Kurt J. Varner, Barry Dellinger, Brian M. Latimer, Tammy R. Dugas

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Particulate matter (PM) is emitted during thermal decomposition of waste. During this process, aromatic compounds chemisorb to the surface of metal-oxide-containing PM, forming a surface-stabilized environmentally persistent free radical (EPFR). We hypothesized that EPFR-containing PM redox cycle to produce ROS and that this redox cycle is maintained in biological environments. To test our hypothesis, we incubated model EPFRs with the fluorescent probe dihydrorhodamine (DHR). Marked increases in DHR fluorescence were observed. Using a more specific assay, hydroxyl radicals ( OH) were also detected, and their level was further increased by cotreatment with thiols or ascorbic acid (AA), known components of epithelial lining fluid. Next, we incubated our model EPFR in bronchoalveolar lavage fluid (BALF) or serum. Detection of EPFRs and OH verified that PM generate ROS in biological fluids. Moreover, incubation of pulmonary epithelial cells with EPFR-containing PM increased OH levels compared to those in PM lacking EPFRs. Finally, measurements of oxidant injury in neonatal rats exposed to EPFRs by inhalation suggested that EPFRs induce an oxidant injury within the lung lining fluid and that the lung responds by increasing antioxidant levels. In summary, our EPFR-containing PM redox cycle to produce ROS, and these ROS are maintained in biological fluids and environments. Moreover, these ROS may modulate toxic responses of PM in biological tissues such as the lung.

Original languageEnglish (US)
Pages (from-to)1862-1871
Number of pages10
JournalChemical Research in Toxicology
Volume26
Issue number12
DOIs
StatePublished - Dec 16 2013

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Particulate Matter
Oxidation-Reduction
Free Radicals
Reactive Oxygen Species
Fluids
Linings
Oxidants
Lung
Aromatic compounds
Poisons
Bronchoalveolar Lavage Fluid
Lung Injury
Fluorescent Dyes
Sulfhydryl Compounds
Hydroxyl Radical
Oxides
Inhalation
Ascorbic Acid
Rats
Assays

All Science Journal Classification (ASJC) codes

  • Toxicology

Cite this

Kelley, M. A., Hebert, V. Y., Thibeaux, T. M., Orchard, M. A., Hasan, F., Cormier, S. A., ... Dugas, T. R. (2013). Model combustion-generated particulate matter containing persistent free radicals redox cycle to produce reactive oxygen species. Chemical Research in Toxicology, 26(12), 1862-1871. https://doi.org/10.1021/tx400227s

Model combustion-generated particulate matter containing persistent free radicals redox cycle to produce reactive oxygen species. / Kelley, Matthew A.; Hebert, Valeria Y.; Thibeaux, Taylor M.; Orchard, Mackenzie A.; Hasan, Farhana; Cormier, Stephania A.; Thevenot, Paul T.; Lomnicki, Slawomir M.; Varner, Kurt J.; Dellinger, Barry; Latimer, Brian M.; Dugas, Tammy R.

In: Chemical Research in Toxicology, Vol. 26, No. 12, 16.12.2013, p. 1862-1871.

Research output: Contribution to journalArticle

Kelley, MA, Hebert, VY, Thibeaux, TM, Orchard, MA, Hasan, F, Cormier, SA, Thevenot, PT, Lomnicki, SM, Varner, KJ, Dellinger, B, Latimer, BM & Dugas, TR 2013, 'Model combustion-generated particulate matter containing persistent free radicals redox cycle to produce reactive oxygen species', Chemical Research in Toxicology, vol. 26, no. 12, pp. 1862-1871. https://doi.org/10.1021/tx400227s
Kelley, Matthew A. ; Hebert, Valeria Y. ; Thibeaux, Taylor M. ; Orchard, Mackenzie A. ; Hasan, Farhana ; Cormier, Stephania A. ; Thevenot, Paul T. ; Lomnicki, Slawomir M. ; Varner, Kurt J. ; Dellinger, Barry ; Latimer, Brian M. ; Dugas, Tammy R. / Model combustion-generated particulate matter containing persistent free radicals redox cycle to produce reactive oxygen species. In: Chemical Research in Toxicology. 2013 ; Vol. 26, No. 12. pp. 1862-1871.
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AU - Hebert, Valeria Y.

AU - Thibeaux, Taylor M.

AU - Orchard, Mackenzie A.

AU - Hasan, Farhana

AU - Cormier, Stephania A.

AU - Thevenot, Paul T.

AU - Lomnicki, Slawomir M.

AU - Varner, Kurt J.

AU - Dellinger, Barry

AU - Latimer, Brian M.

AU - Dugas, Tammy R.

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