Modulation of adaptive immunity by different adjuvant-antigen combinations in mice lacking Nod2

Lilian O. Moreira, Amber Smith, Ashley A. DeFreitas, Joseph E. Qualls, Karim C. El Kasmi, Peter J. Murray

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

The mechanisms underlying adjuvant effects are under renewed scrutiny because of the enormous implications for vaccine development. Additionally, new low-toxicity adjuvants are sought to enhance vaccine formulations. Muramyl dipeptide (MDP) is a component of the peptidoglycan polymer and was shown to be an active but low-toxicity component of complete Freund's adjuvant, a powerful adjuvant composed of mycobacteria lysates in an oil emulsion. MDP activates cells primarily via the cytosolic NLR family member Nod2 and is therefore linked to the ability of adjuvants to enhance antibody production. Accordingly, we tested the adjuvant properties of the MDP-Nod2 pathway. We found that MDP, compared to the TLR agonist lipopolysaccharide, has minimal adjuvant properties for antibody production under a variety of immunization conditions. We also observed that the oil emulsion incomplete Freund's adjuvant (IFA) supplanted the requirements for the TLR pathway independent of the antigen. Surprisingly, we observed that Nod2 was required for an optimal IgG1 and IgG2c response in the absence of exogenous TLR or NLR agonists. Collectively, our results argue that oil emulsions deserve greater attention for their immunostimulatory properties.

Original languageEnglish (US)
Pages (from-to)5808-5813
Number of pages6
JournalVaccine
Volume26
Issue number46
DOIs
StatePublished - Oct 29 2008

Fingerprint

Acetylmuramyl-Alanyl-Isoglutamine
Adaptive Immunity
adjuvants
Emulsions
antigens
Antigens
Oils
dipeptides
mice
Antibody Formation
Vaccines
emulsions
Aptitude
Freund's Adjuvant
Peptidoglycan
antibody formation
Mycobacterium
oils
agonists
Lipopolysaccharides

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Moreira, L. O., Smith, A., DeFreitas, A. A., Qualls, J. E., El Kasmi, K. C., & Murray, P. J. (2008). Modulation of adaptive immunity by different adjuvant-antigen combinations in mice lacking Nod2. Vaccine, 26(46), 5808-5813. https://doi.org/10.1016/j.vaccine.2008.08.038

Modulation of adaptive immunity by different adjuvant-antigen combinations in mice lacking Nod2. / Moreira, Lilian O.; Smith, Amber; DeFreitas, Ashley A.; Qualls, Joseph E.; El Kasmi, Karim C.; Murray, Peter J.

In: Vaccine, Vol. 26, No. 46, 29.10.2008, p. 5808-5813.

Research output: Contribution to journalArticle

Moreira, LO, Smith, A, DeFreitas, AA, Qualls, JE, El Kasmi, KC & Murray, PJ 2008, 'Modulation of adaptive immunity by different adjuvant-antigen combinations in mice lacking Nod2', Vaccine, vol. 26, no. 46, pp. 5808-5813. https://doi.org/10.1016/j.vaccine.2008.08.038
Moreira, Lilian O. ; Smith, Amber ; DeFreitas, Ashley A. ; Qualls, Joseph E. ; El Kasmi, Karim C. ; Murray, Peter J. / Modulation of adaptive immunity by different adjuvant-antigen combinations in mice lacking Nod2. In: Vaccine. 2008 ; Vol. 26, No. 46. pp. 5808-5813.
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