Modulation of murine hepatic lipase activity by exogenous and endogenous Kupffer-cell activation

D. B. Magilavy, R. Zhan, Dennis Black

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Deficiency of hepatic lipase (HL) may play a role in the lipoprotein abnormalities in chronic inflammatory states which are characterized by reticuloendothelial-system activation and cytokine release. HL triacylglycerol hydrolase activity was measured in heparin perfusates of livers from autoimmune MRL/1pr mice, which spontaneously develop a condition closely resembling human lupus erythematosis and exhibit spontaneous Kupffer-cell activation after 8 weeks of age, as well as from normal mice treated with Corynebacterium parvum or polyinosinic-polycytidylic acid complex [poly(I C)] to induce Kupffer-cell activation. HL activity in MRL/1pr mice older than 8 weeks was 29.5 % (P = 0.002) of that in age-matched control MRL/ + + mice. Treatment of normal mice with C. parvum or poly(I C) resulted in HL activities 18. 6 % (P = O.004) and 13.1 % (P = 0.007) respectively of untreated controls. Northern-blot hybridization of liver poly(A)+ RNA showed no differences in HL mRNA abundance in MRL/ + + mice compared with the MRL/1pr autoimmune strain after 8 weeks of age, or in normal control mice compared with those treated with C. parvum, indicating attenuation of HL activity at the translational or post-translational-level. Deficiency of-this enzyme may represent one of the mechanisms contributing to the dyslipoproteinaemia of autoimmune disease and chronic infection.

Original languageEnglish (US)
Pages (from-to)249-252
Number of pages4
JournalBiochemical Journal
Volume292
Issue number1
DOIs
StatePublished - Jan 1 1993

Fingerprint

Kupffer Cells
Lipase
Chemical activation
Modulation
Liver
Propionibacterium acnes
Poly C
Poly I-C
Messenger RNA
Lipases
Mononuclear Phagocyte System
Lipoproteins
Heparin
Dyslipidemias
Northern Blotting
Cytokines
Autoimmune Diseases
Enzymes
Infection

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Modulation of murine hepatic lipase activity by exogenous and endogenous Kupffer-cell activation. / Magilavy, D. B.; Zhan, R.; Black, Dennis.

In: Biochemical Journal, Vol. 292, No. 1, 01.01.1993, p. 249-252.

Research output: Contribution to journalArticle

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