Modulation of virulence gene expression in Staphylococcus aureus by interleukin-1β

Novel implications in bacterial pathogenesis

Siva Kanangat, Arnold Postlethwaite, Shaleen Cholera, Latonya Williams, Dennis Schaberg

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The effect of IL-1β on Staphylococcus aureus was investigated in terms of mRNA expression profile of bicomponent leukotoxins (Luk ED, Luk PV, HlgA, and HlgCB) as well as microbial surface components recognizing adhesive matrix molecules (MSCRAMMs). Upon exposure to higher concentrations of IL-1β, S. aureus expressed significantly higher levels of MSCRAMMs mRNA {fibronectin-binding protein (FnBp), fibrinogen-binding protein or clumping factor (Clf), and collagen-binding protein (Cna)} and had significantly lower expression of mRNAs for bicomponent leukotoxins. Sequential in vitro passing of S. aureus in the absence of rhIL-1β resulted in reduced binding to rhIL-1β resulted in lack of significant changes in virulence gene expression upon exposure to low or high concentrations of rhIL-1β. It is possible that IL-1β modulates the pathogenic potential of S. aureus by altering its virulence gene expression to adapt to the host's inflammatory micromilieu. The ability to express higher levels of MSCRAMMs and low levels of leukotoxins might contribute towards the successful invasion and persistence of S. aureus in chronic inflammatory conditions. Determination of the mechanisms of IL-1-induced alterations in S. aureus gene expression may lead to the identification of novel therapeutic targets against this ever-evolving opportunistic pathogen.

Original languageEnglish (US)
Pages (from-to)408-415
Number of pages8
JournalMicrobes and Infection
Volume9
Issue number3
DOIs
StatePublished - Mar 1 2007

Fingerprint

Interleukin-1
Virulence
Staphylococcus aureus
Gene Expression
Adhesives
Carrier Proteins
Messenger RNA
Fibronectins
Protein Binding
Fibrinogen
Collagen
leukotoxin

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Infectious Diseases

Cite this

Modulation of virulence gene expression in Staphylococcus aureus by interleukin-1β : Novel implications in bacterial pathogenesis. / Kanangat, Siva; Postlethwaite, Arnold; Cholera, Shaleen; Williams, Latonya; Schaberg, Dennis.

In: Microbes and Infection, Vol. 9, No. 3, 01.03.2007, p. 408-415.

Research output: Contribution to journalArticle

Kanangat, Siva ; Postlethwaite, Arnold ; Cholera, Shaleen ; Williams, Latonya ; Schaberg, Dennis. / Modulation of virulence gene expression in Staphylococcus aureus by interleukin-1β : Novel implications in bacterial pathogenesis. In: Microbes and Infection. 2007 ; Vol. 9, No. 3. pp. 408-415.
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