Modulatory effect of copper on nonenzymatic antioxidants in freshwater fish Channa punctatus (Bloch.)

Suhel Parvez, Iqbal Sayeed, Suwarna Pandey, Ausaf Ahmad, Bilal Hafeez, Rizwanul Haque, Iqbal Ahmad, Sheikh Raisuddin

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Effect of the low level of copper exposure on nonenzymatic antioxidants was studied in a freshwater fish Channa punctatus (Bloch.). Fish were exposed to cupric chloride at the concentration of 10 ppb for 4 wk (28 d) in a static culture condition. Copper significantly (p < 0.001) increased the serum ceruloplasmin level and total iron-binding capacity. A significant (p < 0.05) increase in reduced glutathione level was recorded in all of the tissues. With regard to nonprotein thiols, copper decreased their level in the liver, but increased it in the gill. The protein-bound thiols remained unaltered except for an increase in the liver. Metallothionein (MT) induction was observed in liver only. Copper exposure had no significant effect on the ascorbic acid level and induced no lipid peroxidation over control values. It is suggested that by modulating the ceruloplasmin level, copper indirectly protects the fish, as it facilitates conversion of pro-oxidant iron to nonoxidant iron. It also induces an array of antioxidants that may be beneficial to fish in the case of oxidative stress resulting from chemical pollutants.

Original languageEnglish (US)
Pages (from-to)237-248
Number of pages12
JournalBiological Trace Element Research
Volume93
Issue number1-3
DOIs
StatePublished - Jun 1 2003

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Fresh Water
Fish
Copper
Fishes
Antioxidants
Liver
Ceruloplasmin
Iron
Sulfhydryl Compounds
Oxidative stress
Metallothionein
Lipid Peroxidation
Ascorbic Acid
Glutathione
Reactive Oxygen Species
Oxidative Stress
Tissue
Lipids
Serum
Proteins

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical
  • Inorganic Chemistry

Cite this

Modulatory effect of copper on nonenzymatic antioxidants in freshwater fish Channa punctatus (Bloch.). / Parvez, Suhel; Sayeed, Iqbal; Pandey, Suwarna; Ahmad, Ausaf; Hafeez, Bilal; Haque, Rizwanul; Ahmad, Iqbal; Raisuddin, Sheikh.

In: Biological Trace Element Research, Vol. 93, No. 1-3, 01.06.2003, p. 237-248.

Research output: Contribution to journalArticle

Parvez, Suhel ; Sayeed, Iqbal ; Pandey, Suwarna ; Ahmad, Ausaf ; Hafeez, Bilal ; Haque, Rizwanul ; Ahmad, Iqbal ; Raisuddin, Sheikh. / Modulatory effect of copper on nonenzymatic antioxidants in freshwater fish Channa punctatus (Bloch.). In: Biological Trace Element Research. 2003 ; Vol. 93, No. 1-3. pp. 237-248.
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