Molecular characterization of dopamine receptors

M. D. Bates, J. A. Gingrich, J. R. Bunzow, P. Falardeau, A. Dearry, Susan Senogles, O. Civelli, M. G. Caron

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The D1 and D2 dopamine receptors have been biochemically characterized using specific probes based on the subtype selective antagonists SCH 23390 and spiperone, respectively. The D2 dopamine receptor was identified from several tissues by photoaffinity labeling and was purified from bovine anterior pituitary to homogeneity using a combination of affinity, lectin and hydroxylapatite chromatography. A complementary DNA (cDNA) encoding a rat brain D2 dopamine receptor has been cloned via low stringency hybridization using a portion of the β2-adrenergic receptor gene as a probe. Photoaffinity crosslinking and affinity chromatography have also been used to identify and purify the rat brain D1 dopamine receptor.

Original languageEnglish (US)
JournalAmerican journal of hypertension
Volume3
Issue number6 II SUPPL.
StatePublished - Jan 1 1990

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Dopamine D2 Receptors
Dopamine Receptors
Dopamine D1 Receptors
Spiperone
Brain
Durapatite
Affinity Chromatography
Lectins
Adrenergic Receptors
Chromatography
Complementary DNA
Genes

All Science Journal Classification (ASJC) codes

  • Internal Medicine

Cite this

Bates, M. D., Gingrich, J. A., Bunzow, J. R., Falardeau, P., Dearry, A., Senogles, S., ... Caron, M. G. (1990). Molecular characterization of dopamine receptors. American journal of hypertension, 3(6 II SUPPL.).

Molecular characterization of dopamine receptors. / Bates, M. D.; Gingrich, J. A.; Bunzow, J. R.; Falardeau, P.; Dearry, A.; Senogles, Susan; Civelli, O.; Caron, M. G.

In: American journal of hypertension, Vol. 3, No. 6 II SUPPL., 01.01.1990.

Research output: Contribution to journalArticle

Bates, MD, Gingrich, JA, Bunzow, JR, Falardeau, P, Dearry, A, Senogles, S, Civelli, O & Caron, MG 1990, 'Molecular characterization of dopamine receptors', American journal of hypertension, vol. 3, no. 6 II SUPPL..
Bates MD, Gingrich JA, Bunzow JR, Falardeau P, Dearry A, Senogles S et al. Molecular characterization of dopamine receptors. American journal of hypertension. 1990 Jan 1;3(6 II SUPPL.).
Bates, M. D. ; Gingrich, J. A. ; Bunzow, J. R. ; Falardeau, P. ; Dearry, A. ; Senogles, Susan ; Civelli, O. ; Caron, M. G. / Molecular characterization of dopamine receptors. In: American journal of hypertension. 1990 ; Vol. 3, No. 6 II SUPPL.
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