Molecular cloning and characterization of oleoyl-ACP thioesterase gene from Brassica juncea

Nawajes Mandal, I. M. Santha, M. L. Lodha, S. L. Mehta

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Oleoyl-ACP thioesterase (TE) is the chain length-determining enzyme in de novo biosynthesis of oleic acid. For cloning the gene encoding oleoyl-ACP TE from Brassica juncea, a PCR-amplified DNA probe was developed with the primers designed from the known sequences available in the Genbank. It was used to screen a genomic library of B. juncea constructed inλEMBL 3 to get the complete gene. A 4.0 kb Bam HI fragment from the clone λ5.12 and hybridizing to the probe was sequenced. A stretch of 2606 bp genomic sequence was found to contain an ORF of 1101 bp with ∼0.4 kb untranslated regions at both the ends and 5 introns of varied lengths. By homology analysis, the ORF was found to encode an oleoyl-ACP thioesterase gene. The promoter region contains major cis elements required for transcription.

Original languageEnglish (US)
Pages (from-to)27-31
Number of pages5
JournalJournal of Plant Biochemistry and Biotechnology
Volume11
Issue number1
DOIs
StatePublished - Jan 1 2002
Externally publishedYes

Fingerprint

Mustard Plant
Molecular Cloning
Brassica juncea
open reading frames
molecular cloning
Open Reading Frames
DNA probes
genomic libraries
Genes
Untranslated Regions
oleic acid
introns
genes
Genomic Library
transcription (genetics)
Nucleic Acid Databases
DNA Probes
promoter regions
biosynthesis
Oleic Acid

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Molecular cloning and characterization of oleoyl-ACP thioesterase gene from Brassica juncea. / Mandal, Nawajes; Santha, I. M.; Lodha, M. L.; Mehta, S. L.

In: Journal of Plant Biochemistry and Biotechnology, Vol. 11, No. 1, 01.01.2002, p. 27-31.

Research output: Contribution to journalArticle

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