Molecular cloning and expression of rat torsinA in the normal and genetically dystonic (dt) rat

Philipp Ziefer, Joanne Leung, Tara Razzano, Christo Shalish, Mark Ledoux, Joan F. Lorden, Laurie Ozelius, Xandra O. Breakefield, David G. Standaert, Sarah J. Augood

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Deletions within the TOR1A gene cause early-onset (DYT1) torsion dystonia. We have cloned and sequenced the rat cDNA homologue of TOR1A and found a 91% identity with the human sequence. Northern blot analysis detects a single transcript of approximately 1.5 kb. In situ hybridization reveals a widespread distribution of torsinA mRNA within brain. No mutations were identified in the coding region of the gene in the genetically dystonic (dt) rat.

Original languageEnglish (US)
Pages (from-to)132-135
Number of pages4
JournalMolecular Brain Research
Volume101
Issue number1-2
DOIs
StatePublished - May 30 2002

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Molecular Cloning
Northern Blotting
Genes
In Situ Hybridization
Complementary DNA
Messenger RNA
Mutation
Brain
Dystonia musculorum deformans type 1

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Molecular cloning and expression of rat torsinA in the normal and genetically dystonic (dt) rat. / Ziefer, Philipp; Leung, Joanne; Razzano, Tara; Shalish, Christo; Ledoux, Mark; Lorden, Joan F.; Ozelius, Laurie; Breakefield, Xandra O.; Standaert, David G.; Augood, Sarah J.

In: Molecular Brain Research, Vol. 101, No. 1-2, 30.05.2002, p. 132-135.

Research output: Contribution to journalArticle

Ziefer, P, Leung, J, Razzano, T, Shalish, C, Ledoux, M, Lorden, JF, Ozelius, L, Breakefield, XO, Standaert, DG & Augood, SJ 2002, 'Molecular cloning and expression of rat torsinA in the normal and genetically dystonic (dt) rat', Molecular Brain Research, vol. 101, no. 1-2, pp. 132-135. https://doi.org/10.1016/S0169-328X(02)00176-6
Ziefer, Philipp ; Leung, Joanne ; Razzano, Tara ; Shalish, Christo ; Ledoux, Mark ; Lorden, Joan F. ; Ozelius, Laurie ; Breakefield, Xandra O. ; Standaert, David G. ; Augood, Sarah J. / Molecular cloning and expression of rat torsinA in the normal and genetically dystonic (dt) rat. In: Molecular Brain Research. 2002 ; Vol. 101, No. 1-2. pp. 132-135.
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