Molecular identification of a bronopol tolerant strain of Saprolegnia australis causing egg and fry mortality in farmed brown trout, Salmo trutta

Svetlana Rezinciuc, Jose Vladimir Sandoval-Sierra, Javier Diéguez-Uribeondo

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Some species of the genus Saprolegnia, such as Saprolegnia diclina and Saprolegnia ferax are responsible for devastating infections on salmonid eggs. Members of this group cause saprolegniasis, a disease resulting in considerable economic losses in aquaculture. Although both S. diclina and S. ferax have received much attention, the role of other Saprolegnia species in infecting fish eggs is less known. For this purpose, we have investigated the aetiology of chronic egg mortality events occurring in farmed brown trout, Salmo trutta. A total of 48 isolates were obtained from eggs with signs of infection as well as from water samples. A molecular analysis based on nrDNA internal transcribed spacer (ITS) operational taxonomic units indicated that the majority of the isolates correspond to Saprolegnia australis. All isolates of S. australis exhibited the same random amplified polymorphic DNA (RAPD) band patterns suggesting that a single strain is implicated in egg infections. The isolates followed Koch postulates using trout eggs and fry. Under standard concentrations of bronopol commonly used in farms, these isolates could grow, but not sporulate. However, both growth and sporulation were recovered when treatment was removed. This study shows that S. australis can infect and kill salmon eggs, and helps in defining oomycetes core pathogens.

Original languageEnglish (US)
Pages (from-to)591-600
Number of pages10
JournalFungal Biology
Volume118
Issue number7
DOIs
StatePublished - Jan 1 2014

Fingerprint

Saprolegnia
fish roe
Trout
Salmo trutta
fish fry
Eggs
Ovum
egg
mortality
Mortality
Saprolegnia ferax
Infection
Oomycetes
saprolegniosis
Aquaculture
infection
Salmon
random amplified polymorphic DNA technique
fish eggs
sporulation

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Infectious Diseases

Cite this

Molecular identification of a bronopol tolerant strain of Saprolegnia australis causing egg and fry mortality in farmed brown trout, Salmo trutta. / Rezinciuc, Svetlana; Sandoval-Sierra, Jose Vladimir; Diéguez-Uribeondo, Javier.

In: Fungal Biology, Vol. 118, No. 7, 01.01.2014, p. 591-600.

Research output: Contribution to journalArticle

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