Molecular Markers in Stored Kidneys Using Perfluorocarbon-based Preservation Solution: Preliminary Results

Daniel Maluf, Valeria Mas, K. Yanek, J. J. Stone, R. Weis, D. Massey, B. Spiess, M. P. Posner, R. A. Fisher

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background: Delayed graft function (DGF) is a problem in kidney transplantation and cold ischemia has been identified as a risk factor. Perfluorocarbons (PFC) have an enhanced ability to dissolve and release oxygen. We evaluated histologically and a number of molecular changes induced by ischemia in stored kidneys with University of Wisconsin (UW) and PFC-based preservation solutions (PFC-UW). Materials and methods: ACI rats were used as kidney donors. UW (control group) or PFC-UW (study group) preservation solutions were used for kidney perfusion. All kidneys were stored at 4°C for 12, 24, and 36 hours. After this time, intragraft histologic evaluation as well as mRNA HO-1 and iNOS levels were also analyzed. Results: In the kidneys stored at 24 hours, mRNA HO-1 levels were elevated in the study group when compared with the control and mRNA iNOS was decreased. Conclusion: We observed overexpression of HO-1 and underexpression of iNOS in the kidney tissue stored with PFC-UW solution at 24 hours. These preliminary data suggest that increasing oxygen delivery by PFC added to the perfusion solution triggers cytoprotective mechanism in kidney transplantation.

Original languageEnglish (US)
Pages (from-to)1243-1246
Number of pages4
JournalTransplantation Proceedings
Volume38
Issue number5
DOIs
StatePublished - Jun 1 2006
Externally publishedYes

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Fluorocarbons
Kidney
Kidney Transplantation
Messenger RNA
Perfusion
Inbred ACI Rats
Oxygen
Delayed Graft Function
Cold Ischemia
Ischemia
Control Groups

All Science Journal Classification (ASJC) codes

  • Surgery
  • Transplantation

Cite this

Molecular Markers in Stored Kidneys Using Perfluorocarbon-based Preservation Solution : Preliminary Results. / Maluf, Daniel; Mas, Valeria; Yanek, K.; Stone, J. J.; Weis, R.; Massey, D.; Spiess, B.; Posner, M. P.; Fisher, R. A.

In: Transplantation Proceedings, Vol. 38, No. 5, 01.06.2006, p. 1243-1246.

Research output: Contribution to journalArticle

Maluf, Daniel ; Mas, Valeria ; Yanek, K. ; Stone, J. J. ; Weis, R. ; Massey, D. ; Spiess, B. ; Posner, M. P. ; Fisher, R. A. / Molecular Markers in Stored Kidneys Using Perfluorocarbon-based Preservation Solution : Preliminary Results. In: Transplantation Proceedings. 2006 ; Vol. 38, No. 5. pp. 1243-1246.
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